Literature summary for 6.1.1.27 extracted from

Liu, Y.; Nakamura, A.; Nakazawa, Y.; Asano, N.; Ford, K.A.; Hohn, M.J.; Tanaka, I.; Yao, M.; Soell, D.
Ancient translation factor is essential for tRNA-dependent cysteine biosynthesis in methanogenic archaea (2014), Proc. Natl. Acad. Sci. USA, 111, 10520-10525 .

Search for more literature for 6.1.1.27

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta 2(DE3) cells	Methanococcus maripaludis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0116	-	tRNACys	at pH 7.5 and 60°C	Methanococcus maripaludis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
270000	-	gel filtration	Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + O-phospho-L-serine + tRNACys	Methanococcus maripaludis	-	AMP + diphosphate + O-phospho-L-seryl-tRNACys	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanococcus maripaludis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
TALON metal affinity resin column chromatography	Methanococcus maripaludis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + O-phospho-L-serine + tRNACys	-	Methanococcus maripaludis	AMP + diphosphate + O-phospho-L-seryl-tRNACys	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 60000, SDS-PAGE	Methanococcus maripaludis

Synonyms

Synonyms	Comment	Organism
phosphoseryl-tRNA synthetase	-	Methanococcus maripaludis
SepRS	-	Methanococcus maripaludis
sepS	-	Methanococcus maripaludis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.07	-	tRNACys	at pH 7.5 and 60°C	Methanococcus maripaludis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Methanococcus maripaludis

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Release 2023.1 (January 2023)