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Literature summary for 6.1.1.27 extracted from

  • Chen, M.; Nakazawa, Y.; Kubo, Y.; Asano, N.; Kato, K.; Tanaka, I.; Yao, M.
    Crystallographic analysis of a subcomplex of the transsulfursome with tRNA for Cys-tRNACys synthesis (2016), Acta Crystallogr. Sect. F, 72, 569-572 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.27

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834(DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M MES pH 5.3, 2.37 M ammonium sulfate Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + O-phospho-L-serine + tRNACys Methanocaldococcus jannaschii
-
 AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q59054
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + O-phospho-L-serine + tRNACys
-
 Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Synonyms

Synonyms Comment Organism
CysRS
-
 Methanocaldococcus jannaschii
Cysteinyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
O-phosphoseryl-tRNA synthetase
-
 Methanocaldococcus jannaschii
SepRS
-
 Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanocaldococcus jannaschii