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Literature summary for 6.1.1.26 extracted from

  • Ko, J.H.; Wang, Y.S.; Nakamura, A.; Guo, L.T.; Soell, D.; Umehara, T.
    Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function (2013), FEBS Lett., 587, 3243-3248.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
A302F/Y306L/N346T/C348F/Y384L the mutant acylates tRNAPyl with L-phenylalanine Escherichia coli
A302L/Y306M/N346S/C348L/Y384L the mutant acylates tRNAPyl with L-phenylalanine Escherichia coli
N346A/C348L the mutant acylates tRNAPyl with L-phenylalanine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 4 L-phenylalanine mutant enzyme A302L/Y306M/N346S/C348L/Y384L, pH and temperature not specified in the publication Escherichia coli
21
-
 L-phenylalanine mutant enzyme N346A/C348L, pH and temperature not specified in the publication Escherichia coli
22.8
-
 L-phenylalanine mutant enzyme A302F/Y306L/N346T/C348F/Y384L, pH and temperature not specified in the publication Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl Escherichia coli
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
additional information Escherichia coli the wild type enzyme shows no activity with L-phenylalanine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPyl mutant enzymes N346A/C348L, A302L/Y306M/N346S/C348L/Y384L, and A302F/Y306L/N346T/C348F/Y384L use L-phenylalanine as substrate Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPyl
-
 ?
ATP + L-pyrrolysine + tRNAPyl
-
 Escherichia coli AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
additional information the wild type enzyme shows no activity with L-phenylalanine Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
PylRS
-
 Escherichia coli
pyrrolysyl-tRNA synthetase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037
-
 L-phenylalanine mutant enzyme N346A/C348L, pH and temperature not specified in the publication Escherichia coli
0.062
-
 L-phenylalanine mutant enzyme A302L/Y306M/N346S/C348L/Y384L, pH and temperature not specified in the publication Escherichia coli
0.075
-
 L-phenylalanine mutant enzyme A302F/Y306L/N346T/C348F/Y384L, pH and temperature not specified in the publication Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli