Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant PylRS in Escherichia coli | Methanosarcina mazei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | evolving a PylRS mutant that specifically acylates tRNAPylCUA with L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine by standard positive and negative selections of the pRS1 plasmid library in Escherichia coli. The pRS1 plasmid library contains the Methanosarcina mazei PylRS gene with randomization at six active-site residues, L305, Y306, L309, N346, C348, and W417, method, overview | Methanosarcina mazei |
additional information | usage of evolved pyrrolysyl-tRNA synthetase-tRNAPylCUA pairs for genetic incorporation of L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine into proteins at amber mutation sites in Escherichia coli | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanosarcina mazei | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
pyrrolysyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
pyrrolysyl-tRNA synthetase | - |
Methanosarcina mazei |