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Literature summary for 6.1.1.26 extracted from

  • Polycarpo, C.R.; Herring, S.; Berube, A.; Wood, J.L.; Soll, D.; Ambrogelly, A.
    Pyrrolysine analogues as substrates for pyrrolysyl-tRNA synthetase (2006), FEBS Lett., 580, 6695-6700.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
functional co-expression with tRNAPyl in Escherichia coli, the recombinant enzyme is active with substrate analogues N-epsilon-D-prolyl-L-lysine and N-epsilon-cyclopentyloxycarbonyl-L-lysine Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 pyrrolysine pH 7.2, 37°C, recombinant enzyme Methanosarcina barkeri
0.5
-
 N-epsilon-D-prolyl-L-lysine pH 7.2, 37°C, recombinant enzyme Methanosarcina barkeri
0.67
-
 N-epsilon-cyclopentyloxycarbonyl-L-lysine pH 7.2, 37°C, recombinant enzyme Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl Methanosarcina barkeri Pyl-tRNAPyl insertion at UAG, a specialized mRNA motif is not essential for stopcodon recoding, unlike for selenocysteine incorporation AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
additional information Methanosarcina barkeri while pyrrolysine is the natural substrate of PylRS, lysine is not recognized by the enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
 gene pylS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina barkeri AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
ATP + L-pyrrolysine + tRNAPyl Pyl-tRNAPyl insertion at UAG, a specialized mRNA motif is not essential for stopcodon recoding, unlike for selenocysteine incorporation Methanosarcina barkeri AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
ATP + N-epsilon-cyclopentyloxycarbonyl-L-lysine + tRNAPyl
-
 Methanosarcina barkeri AMP + diphosphate + N-epsilon-cyclopentyloxycarbonyl-L-lysyl-tRNAPyl
-
 ?
ATP + N-epsilon-D-prolyl-L-lysine + tRNAPyl
-
 Methanosarcina barkeri AMP + diphosphate + N-epsilon-D-prolyl-L-lysyl-tRNAPyl
-
 ?
additional information while pyrrolysine is the natural substrate of PylRS, lysine is not recognized by the enzyme Methanosarcina barkeri ?
-
 ?

Synonyms

Synonyms Comment Organism
PylRS
-
 Methanosarcina barkeri
pyrrolysyl-tRNA synthetase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanosarcina barkeri