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Literature summary for 6.1.1.24 extracted from

  • Pelchat, M.; Lacoste, L.; Yang, F.; Lapointe, J.
    Overproduction of the Bacillus subtilis glutamyl-tRNA synthetase in its host and its toxicity to Escherichia coli (1998), Can. J. Microbiol., 44, 378-381.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
the gene is cloned with its sigmaA promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and bacillus subtilis. Transformation of Bacillus subtilis with this recombinant plasmidleads to a 30fold increase of glutamyl-tRNA synthetase specific activity in crude extracts. Transformation of Escherichia coli with this plasmid gives no recombinants. The presence of Bacillus subtilis glutamyl-tRNA synthetase is lethal for Escherichia coli, probably because this enzyme glutamylates tRNA1Gln in vivo as it does in vitro Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGln
-
 Bacillus subtilis AMP + diphosphate + Glu-tRNAGln
-
 ?
ATP + L-glutamate + tRNAGlu
-
 Bacillus subtilis AMP + diphosphate + Glu-tRNAGlu
-
 ?