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Literature summary for 6.1.1.23 extracted from

  • Nair, N.; Raff, H.; Islam, M.T.; Feen, M.; Garofalo, D.M.; Sheppard, K.
    The Bacillus subtilis and Bacillus halodurans aspartyl-tRNA synthetases retain recognition of tRNAAsn (2016), J. Mol. Biol., 428, 618-630 .
    View publication on PubMed
Search for more literature for 6.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JF448 cells Borreliella burgdorferi
expressed in Escherichia coli JF448 cells Halalkalibacterium halodurans
expressed in Escherichia coli JF448 cells Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 tRNAAsn at pH 8.0 and 37°C Halalkalibacterium halodurans
0.0015
-
 tRNAAsp at pH 8.0 and 37°C Halalkalibacterium halodurans
0.0016
-
 tRNAAsp at pH 7.2 and 37°C Bacillus subtilis
0.0016
-
 tRNAAsn at pH 7.2 and 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Borreliella burgdorferi
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp Halalkalibacterium halodurans
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp Bacillus subtilis
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp Bacillus subtilis 168
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O32038
-
-
Bacillus subtilis 168 O32038
-
-
Borreliella burgdorferi
-
-
-
Halalkalibacterium halodurans Q9KDG1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsn about 20% activity compared to tRNAAsp Halalkalibacterium halodurans AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsn about 20% activity compared to tRNAAsp Bacillus subtilis AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsn about 60% activity compared to tRNAAsp Borreliella burgdorferi AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsn about 20% activity compared to tRNAAsp Bacillus subtilis 168 AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Borreliella burgdorferi AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Halalkalibacterium halodurans AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Bacillus subtilis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp 100% activity Borreliella burgdorferi AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp 100% activity Halalkalibacterium halodurans AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp 100% activity Bacillus subtilis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Bacillus subtilis 168 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp 100% activity Bacillus subtilis 168 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
additional information the enzyme shows no activity with tRNAGln Borreliella burgdorferi ?
-
 ?
additional information the enzyme shows no activity with tRNAGln Halalkalibacterium halodurans ?
-
 ?
additional information the enzyme shows no activity with tRNAGln Bacillus subtilis ?
-
 ?
additional information the enzyme shows no activity with tRNAGln Bacillus subtilis 168 ?
-
 ?

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
-
 Borreliella burgdorferi
Aspartyl-tRNA synthetase
-
 Halalkalibacterium halodurans
Aspartyl-tRNA synthetase
-
 Bacillus subtilis
AspRS
-
 Borreliella burgdorferi
AspRS
-
 Halalkalibacterium halodurans
AspRS
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 tRNAAsn at pH 8.0 and 37°C Halalkalibacterium halodurans
0.058
-
 tRNAAsn at pH 7.2 and 37°C Bacillus subtilis
0.115
-
 tRNAAsp at pH 8.0 and 37°C Halalkalibacterium halodurans
0.36
-
 tRNAAsp at pH 7.2 and 37°C Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Borreliella burgdorferi
ATP
-
 Halalkalibacterium halodurans
ATP
-
 Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 tRNAAsn at pH 8.0 and 37°C Halalkalibacterium halodurans
40
-
 tRNAAsn at pH 7.2 and 37°C Bacillus subtilis
80
-
 tRNAAsp at pH 8.0 and 37°C Halalkalibacterium halodurans
220
-
 tRNAAsp at pH 7.2 and 37°C Bacillus subtilis