Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
tRNAAsp | at pH 7.2 and 37°C | Staphylococcus aureus | |
0.0031 | - |
tRNAAsn | at pH 7.2 and 37°C | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Staphylococcus aureus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Staphylococcus aureus FPR3757 | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus FPR3757 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | - |
Staphylococcus aureus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsn | - |
Staphylococcus aureus FPR3757 | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Staphylococcus aureus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Staphylococcus aureus FPR3757 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartyl-tRNA synthetase | - |
Staphylococcus aureus |
AspRS | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
tRNAAsn | at pH 7.2 and 37°C | Staphylococcus aureus | |
0.24 | - |
tRNAAsp | at pH 7.2 and 37°C | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
tRNAAsn | at pH 7.2 and 37°C | Staphylococcus aureus | |
100 | - |
tRNAAsp | at pH 7.2 and 37°C | Staphylococcus aureus |