Cloned (Comment) | Organism |
---|---|
the N-terminal anticodon-binding domain of non-discriminating aspartyl-tRNA synthetase is expressed in Escherichia coli BL21(DE3) cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
N-terminal anticodon-binding domain of non-discriminating aspartyl-tRNA synthetase, hanging drop vapor diffusion method, using 30% (w/v) PEG 4000, 100 mM ammonium sulfate, 100 mM sodium citrate at pH 5.6 | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12870 | - |
1 * 12870, calculated from amino acid sequence | Helicobacter pylori |
15500 | - |
gel filtration | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | Helicobacter pylori | - |
AMP + diphosphate + aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | Helicobacter pylori | - |
AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | - |
Helicobacter pylori | AMP + diphosphate + aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Helicobacter pylori | AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 12870, calculated from amino acid sequence | Helicobacter pylori |
monomer | 1 * 15500, gel filtration | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
ND-AspRS | - |
Helicobacter pylori |
non-discriminating aspartyl-tRNA synthetase | - |
Helicobacter pylori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 42 | the recombinant purified N-terminal anticodon-binding domain of non-discriminating aspartyl-tRNA synthetase is stable up to 42°C | Helicobacter pylori |