Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.23 extracted from

  • Ryckelynck, M.; A Paulus, C.; Frugier, M.
    Post-translational modifications guard yeast from misaspartylation (2008), Biochemistry, 47, 12476-12482.
    View publication on PubMed
Search for more literature for 6.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in YBC-603 cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information AspRS overexpression leads deprivation of the gene's 5'-untranslated region, that is essential for directing mRNA recognition by the protein and initiating the retro-inhibition process, but does not lead to increased tRNAAsn and/or tRNAGlu misaspartylation or the logical consecutive post-translational stress Saccharomyces cerevisiae
R485K a catalytic site mutant Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsx Saccharomyces cerevisiae
-
 AMP + diphosphate + L-aspartyl-tRNAAsx
-
 ?
additional information Saccharomyces cerevisiae the enzyme is downregulated at the post-transcriptional level in a complex retro-inhibition mechanism, the cell equilibrates cellular concentrations of tRNAAsp, AspRS, and its encoding mRNA to hinder AspRS accumulation and avoid misacylation of heterologous tRNAs, proteomic analysis, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information post-translational modifications in the N-terminal extension of AspRS neutralizing the lysine-rich motif contained in this domain Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsx
-
 Saccharomyces cerevisiae AMP + diphosphate + L-aspartyl-tRNAAsx
-
 ?
additional information the enzyme is downregulated at the post-transcriptional level in a complex retro-inhibition mechanism, the cell equilibrates cellular concentrations of tRNAAsp, AspRS, and its encoding mRNA to hinder AspRS accumulation and avoid misacylation of heterologous tRNAs, proteomic analysis, overview Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
-
 Saccharomyces cerevisiae
AspRS
-
 Saccharomyces cerevisiae