Cloned (Comment) | Organism |
---|---|
expression of C-terminally His-tagged enzyme in strain ADD1976 | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-aspartol adenylate | i.e. Asp-ol-AMP, a stable analogue of the natural reaction intermediate L-aspartyl adenylate, biphasic, competitive inhibition, differential inhibition of tRNAAsp and tRNAAsn aspartylation by the enzyme, overview | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Pseudomonas aeruginosa | - |
AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain ADD1976 by nickel affinity chromatography | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-aspartate + tRNAAsx = AMP + diphosphate + L-aspartyl-tRNAAsx | via reaction intermediate L-aspartyl adenylate | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Pseudomonas aeruginosa | AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsx | preparation of recombinant tRNA substrates from Pseudomonas aeruginosa and Saccharomyces cerevisiae, overview, activity with two variants C36U and C38U of yeast tRNAAsp | Pseudomonas aeruginosa | AMP + diphosphate + L-aspartyl-tRNAAsx | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ND-AspRS | - |
Pseudomonas aeruginosa |
nondiscriminating aspartyl-tRNA synthetase | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Pseudomonas aeruginosa | |
0.041 | - |
L-aspartol adenylate | tRNAAsp aspartylation | Pseudomonas aeruginosa | |
0.215 | - |
L-aspartol adenylate | tRNAAsn aspartylation | Pseudomonas aeruginosa |