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Literature summary for 6.1.1.23 extracted from

  • Sato, Y.; Maeda, Y.; Shimizu, S.; Hossain, M.T.; Ubukata, S.; Suzuki, K.; Sekiguchi, T.; Takenaka, A.
    Structure of the nondiscriminating aspartyl-tRNA synthetase from the crenarchaeon Sulfolobus tokodaii strain 7 reveals the recognition mechanism for two different tRNA anticodons (2007), Acta Crystallogr. Sect. D, 63, 1042-1047.
    View publication on PubMed
Search for more literature for 6.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21(DE3) Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, X-ray diffraction structure determination and analysis at 2.3 A resolution Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsn Sulfurisphaera tokodaii dual activity of the ND-AspRS since an asparaginyl-tRNA synthetase is missing in Sulfolobus tokodaii strain 7 AMP + diphosphate + aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsp Sulfurisphaera tokodaii
-
 AMP + diphosphate + aspartyl-tRNAAsp
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in Sulfolobus tokodaii
-
-
-
no activity in Sulfolobus tokodaii 7
-
-
-
Sulfurisphaera tokodaii
-
 a crenarchaeon, strain 7
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Sulfurisphaera tokodaii

Reaction

Reaction Comment Organism Reaction ID
ATP + L-aspartate + tRNAAsx = AMP + diphosphate + L-aspartyl-tRNAAsx substrate anticodon, GUC for aspartate and GUU for asparagine, recognition site structure and mechanism Sulfurisphaera tokodaii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsn dual activity of the ND-AspRS since an asparaginyl-tRNA synthetase is missing in Sulfolobus tokodaii strain 7 Sulfurisphaera tokodaii AMP + diphosphate + aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Sulfurisphaera tokodaii AMP + diphosphate + aspartyl-tRNAAsp
-
 ?

Subunits

Subunits Comment Organism
More structural comparison of the nondiscriminating AspRS with structures of discriminating AspRSs, EC 6.1.1.12, highly conserved catalytic domain, but different tRNA binding site in the N-terminal domain, overview Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
nondiscriminating aspartyl-tRNA synthetase
-
 Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Sulfurisphaera tokodaii