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Literature summary for 6.1.1.20 extracted from

  • Abibi, A.; Ferguson, A.D.; Fleming, P.R.; Gao, N.; Hajec, L.I.; Hu, J.; Laganas, V.A.; McKinney, D.C.; McLeod, S.M.; Prince, D.B.; Shapiro, A.B.; Buurman, E.T.
    The role of a novel auxiliary pocket in bacterial phenylalanyl-tRNA synthetase druggability (2014), J. Biol. Chem., 289, 21651-21662 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2-(3-methoxyphenyl)-5-(trifluoromethyl)-1H-imidazole
-
 Escherichia coli
2-(3-methoxyphenyl)-5-(trifluoromethyl)-1H-imidazole
-
 Pseudomonas aeruginosa
2-[(4-chlorophenoxy)methyl]-N-(2-sulfamoylethyl)-1,3-thiazole-4-carboxamide
-
 Escherichia coli
2-[(4-chlorophenoxy)methyl]-N-(2-sulfamoylethyl)-1,3-thiazole-4-carboxamide
-
 Pseudomonas aeruginosa
2-[(4-chlorophenoxy)methyl]-N-(cyanomethyl)-1,3-thiazole-4-carboxamide
-
 Escherichia coli
2-[(4-chlorophenoxy)methyl]-N-(cyanomethyl)-1,3-thiazole-4-carboxamide
-
 Pseudomonas aeruginosa
2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-(1,1-dioxo-1l6-thiolan-3-yl)acetamide
-
 Escherichia coli
2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-(1,1-dioxo-1l6-thiolan-3-yl)acetamide
-
 Pseudomonas aeruginosa
2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-methylacetamide
-
 Escherichia coli
2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-methylacetamide
-
 Pseudomonas aeruginosa
2-[3-[(4-cyanopyridin-2-yl)amino]phenoxy]-N-methylacetamide
-
 Escherichia coli
2-[3-[(4-cyanopyridin-2-yl)amino]phenoxy]-N-methylacetamide
-
 Pseudomonas aeruginosa
5-(3-methoxyphenyl)-3-(trifluoromethyl)-1H-pyrazole
-
 Escherichia coli
5-(3-methoxyphenyl)-3-(trifluoromethyl)-1H-pyrazole
-
 Pseudomonas aeruginosa
ethyl [3-[3-(difluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
-
 Escherichia coli
ethyl [3-[3-(difluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
-
 Pseudomonas aeruginosa
ethyl [3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
-
 Escherichia coli
ethyl [3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
-
 Pseudomonas aeruginosa
N-(1,1-dioxo-1l6-thiolan-3-yl)-2-[(4-methylphenoxy)methyl]-1,3-thiazole-4-carboxamide
-
 Escherichia coli
N-(1,1-dioxo-1l6-thiolan-3-yl)-2-[(4-methylphenoxy)methyl]-1,3-thiazole-4-carboxamide
-
 Pseudomonas aeruginosa
N-[2-(1H-indol-3-yl)ethyl]-3-[[(1,3-thiazol-2-yl)carbamoyl]amino]benzene-1-sulfonamide
-
 Escherichia coli
N-[2-(1H-indol-3-yl)ethyl]-3-[[(1,3-thiazol-2-yl)carbamoyl]amino]benzene-1-sulfonamide
-
 Pseudomonas aeruginosa
N-[2-[1-(methanesulfonyl)cyclopropyl]ethyl]-2-[3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetamide
-
 Escherichia coli
N-[2-[1-(methanesulfonyl)cyclopropyl]ethyl]-2-[3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetamide
-
 Pseudomonas aeruginosa
N-[3-[4-(pyridin-2-yl)piperazine-1-sulfonyl]phenyl]-N'-1,3-thiazol-2-ylurea
-
 Escherichia coli
N-[3-[4-(pyridin-2-yl)piperazine-1-sulfonyl]phenyl]-N'-1,3-thiazol-2-ylurea
-
 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Escherichia coli
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe Pseudomonas aeruginosa
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe Pseudomonas aeruginosa PAO01
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa PAO01
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Ni2+ chelating column chromatography, Ni-NTA column chromatography and Sephacryl S300 gel filtration Escherichia coli
HiTrap Ni2+ chelating column chromatography, Ni-NTA column chromatography and Sephacryl S300 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe
-
 Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Pseudomonas aeruginosa AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Pseudomonas aeruginosa PAO01 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Synonyms

Synonyms Comment Organism
Phenylalanine-tRNA synthetase
-
 Escherichia coli
Phenylalanyl-tRNA synthetase
-
 Pseudomonas aeruginosa
PheRS
-
 Escherichia coli
PheRS
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Pseudomonas aeruginosa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000022
-
 pH and temperature not specified in the publication Escherichia coli N-[2-(1H-indol-3-yl)ethyl]-3-[[(1,3-thiazol-2-yl)carbamoyl]amino]benzene-1-sulfonamide
0.0000027
-
 pH and temperature not specified in the publication Escherichia coli N-[3-[4-(pyridin-2-yl)piperazine-1-sulfonyl]phenyl]-N'-1,3-thiazol-2-ylurea
0.000013
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa N-[2-(1H-indol-3-yl)ethyl]-3-[[(1,3-thiazol-2-yl)carbamoyl]amino]benzene-1-sulfonamide
0.000022
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa N-[3-[4-(pyridin-2-yl)piperazine-1-sulfonyl]phenyl]-N'-1,3-thiazol-2-ylurea
0.00006
-
 pH and temperature not specified in the publication Escherichia coli 5-(3-methoxyphenyl)-3-(trifluoromethyl)-1H-pyrazole
0.00007
-
 pH and temperature not specified in the publication Escherichia coli N-[2-[1-(methanesulfonyl)cyclopropyl]ethyl]-2-[3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetamide
0.00008
-
 pH and temperature not specified in the publication Escherichia coli ethyl [3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
0.0001
-
 pH and temperature not specified in the publication Escherichia coli 2-[(4-chlorophenoxy)methyl]-N-(cyanomethyl)-1,3-thiazole-4-carboxamide
0.0001
-
 pH and temperature not specified in the publication Escherichia coli 2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-(1,1-dioxo-1l6-thiolan-3-yl)acetamide
0.00019
-
 pH and temperature not specified in the publication Escherichia coli 2-[(4-chlorophenoxy)methyl]-N-(2-sulfamoylethyl)-1,3-thiazole-4-carboxamide
0.00056
-
 pH and temperature not specified in the publication Escherichia coli 2-[3-[(4-cyanopyridin-2-yl)amino]phenoxy]-N-methylacetamide
0.001
-
 pH and temperature not specified in the publication Escherichia coli ethyl [3-[3-(difluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
0.0014
-
 pH and temperature not specified in the publication Escherichia coli 2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-methylacetamide
0.0014
-
 pH and temperature not specified in the publication Escherichia coli N-(1,1-dioxo-1l6-thiolan-3-yl)-2-[(4-methylphenoxy)methyl]-1,3-thiazole-4-carboxamide
0.0042
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa N-[2-[1-(methanesulfonyl)cyclopropyl]ethyl]-2-[3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetamide
0.01
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa 5-(3-methoxyphenyl)-3-(trifluoromethyl)-1H-pyrazole
0.011
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa ethyl [3-[3-(trifluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
0.013
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa 2-[(4-chlorophenoxy)methyl]-N-(cyanomethyl)-1,3-thiazole-4-carboxamide
0.016
-
 pH and temperature not specified in the publication Escherichia coli 2-(3-methoxyphenyl)-5-(trifluoromethyl)-1H-imidazole
0.026
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa 2-[(4-chlorophenoxy)methyl]-N-(2-sulfamoylethyl)-1,3-thiazole-4-carboxamide
0.034
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa 2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-(1,1-dioxo-1l6-thiolan-3-yl)acetamide
0.041
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa 2-[3-[(4-cyanopyridin-2-yl)amino]phenoxy]-N-methylacetamide
0.059
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa ethyl [3-[3-(difluoromethyl)-1H-pyrazol-5-yl]phenoxy]acetate
0.08
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa N-(1,1-dioxo-1l6-thiolan-3-yl)-2-[(4-methylphenoxy)methyl]-1,3-thiazole-4-carboxamide
0.082
-
 pH and temperature not specified in the publication Pseudomonas aeruginosa 2-[3-[(4-chloropyridin-2-yl)amino]phenoxy]-N-methylacetamide
0.2
-
 IC50 above 0.2 mM, pH and temperature not specified in the publication Pseudomonas aeruginosa 2-(3-methoxyphenyl)-5-(trifluoromethyl)-1H-imidazole