Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21-RIL/pET16b produces His6-tagged mtPheRS encoded by the MSF1 gene, the ctPheRS alpha and beta subunits, encoded by the FRS1 and FRS2 genes, respectively, are expressed in tandem from pQE31-FRS-sc in Escherichia coli BL21-RIL cells | Saccharomyces cerevisiae |
Escherichia coli pET21c+ encoding human mtPheRS, FARS2, produces mature His6-tagged wild-type PheRS | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A294G | EcPheRS mutant | Escherichia coli |
A333G | mtPheRS mutant | Saccharomyces cerevisiae |
G458A | ctPheRS mutant | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
L-phenylalanine | wild-type EcPheRS | Escherichia coli | |
0.003 | - |
L-phenylalanine | wild-type ctPheRS | Saccharomyces cerevisiae | |
0.0045 | - |
L-phenylalanine | mutant A294G EcPheRS | Escherichia coli | |
0.005 | - |
L-phenylalanine | wild-type mtPheRS | Saccharomyces cerevisiae | |
0.017 | - |
L-phenylalanine | mutant A333G mtPheRS | Saccharomyces cerevisiae | |
0.233 | - |
L-phenylalanine | mutant G458A ctPheRS | Saccharomyces cerevisiae | |
0.32 | - |
L-tyrosine | mutant A294G EcPheRS | Escherichia coli | |
0.637 | - |
L-tyrosine | wild-type ctPheRS | Saccharomyces cerevisiae | |
0.66 | - |
L-tyrosine | mutant A333G mtPheRS | Saccharomyces cerevisiae | |
1.155 | - |
L-tyrosine | wild-type mtPheRS | Saccharomyces cerevisiae | |
2.2 | - |
L-tyrosine | wild-type EcPheRS | Escherichia coli | |
3 | - |
L-tyrosine | mutant G458A ctPheRS | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | ctPheRS | Saccharomyces cerevisiae | 5737 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | mtPheRS | Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | Escherichia coli | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | Homo sapiens | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-tagged proteins are purified on nickel-nitrilotriacetic acid-agarose | Homo sapiens |
His6-tagged proteins are purified on nickel-nitrilotriacetic acid-agarose | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | - |
Escherichia coli | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-tyrosine + tRNAPhe | - |
Escherichia coli | AMP + diphosphate + L-tyrosyl-tRNAPhe | - |
? | |
ATP + L-tyrosine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-tyrosyl-tRNAPhe | - |
? | |
ATP + L-tyrosine + tRNAPhe | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-tyrosyl-tRNAPhe | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | ctPheRS, alpha and beta-subunits, the alpha-subunits contain the actice site, the beta-subunits harbor the editing site | Saccharomyces cerevisiae |
heterotetramer | EcPheRS, alpha and beta-subunits, the alpha-subunits contain the actice site, the beta-subunits harbor the editing site | Escherichia coli |
monomer | - |
Homo sapiens |
monomer | mtPheRS, alpha-subunit monomer, catalytic domain | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ctPheRS | cytoplasmic type | Saccharomyces cerevisiae |
EcPheRS | - |
Escherichia coli |
mtPheRS | mitochondrial type | Homo sapiens |
mtPheRS | mitochondrial type | Saccharomyces cerevisiae |
Phenylalanyl-tRNA synthetase | - |
Escherichia coli |
Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
Phenylalanyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
PheRS | - |
Escherichia coli |
PheRS | - |
Homo sapiens |
PheRS | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
L-tyrosine | mutant G458S ctPheRS | Saccharomyces cerevisiae | |
5 | - |
L-tyrosine | wild-type mtPheRS | Saccharomyces cerevisiae | |
12.5 | - |
L-tyrosine | mutant A333G mtPheRS | Saccharomyces cerevisiae | |
35 | - |
L-tyrosine | wild-type EcPheRS | Escherichia coli | |
140 | - |
L-phenylalanine | mutant A333G mtPheRS | Saccharomyces cerevisiae | |
180 | - |
L-phenylalanine | wild-type mtPheRS | Saccharomyces cerevisiae | |
184 | - |
L-tyrosine | wild-type ctPheRS | Saccharomyces cerevisiae | |
185 | - |
L-phenylalanine | mutant A294G EcPheRS | Escherichia coli | |
185 | - |
L-tyrosine | mutant A294G EcPheRS | Escherichia coli | |
199 | - |
L-phenylalanine | wild-type EcPheRS | Escherichia coli | |
464 | - |
L-phenylalanine | mutant G45A8 ctPheRS | Saccharomyces cerevisiae | |
603 | - |
L-phenylalanine | wild-type ctPheRS | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Homo sapiens | |
ATP | - |
Saccharomyces cerevisiae |