Cloned (Comment) | Organism |
---|---|
expression of wild-type isolated B3/4 editing domain, and of the editing domain fused to the N-terminus of Escherichia coli iodoTyrRS to generate N-Eed-IYRS, overview | Escherichia coli |
expression of wild-type isolated B3/4 editing domain, and of the editing domain fused to the N-terminus of Escherichia coli iodoTyrRS to generate N-Ped-IYRS, overview | Pyrococcus horikoshii |
expression of wild-type isolated B3/4 editing domain, and of the editing domain fused to the N-terminus of Escherichia coli iodoTyrRS to generate N-Ted-IYRS, overview | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the editing domain of PheRS is transplanted at internal sites into Escherichia coli iodoTyrRS to edit tyrosyl-tRNATyr and thereby improve the overall specificity for 3-iodo-L-tyrosine, overview | Thermus thermophilus |
additional information | the editing domain of PheRS is transplanted at internal sites into Escherichia coli iodoTyrRS to edit tyrosyl-tRNATyr and thereby improve the overall specificity for 3-iodo-L-tyrosine, overview | Escherichia coli |
additional information | the editing domain of PheRS is transplanted at internal sites into Escherichia coli iodoTyrRS to edit tyrosyl-tRNATyr and thereby improve the overall specificity for 3-iodo-L-tyrosine, overview | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Thermus thermophilus | |
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | Thermus thermophilus | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | Escherichia coli | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | Pyrococcus horikoshii | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pyrococcus horikoshii | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | - |
Thermus thermophilus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | - |
Escherichia coli | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | - |
Pyrococcus horikoshii | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
additional information | editing activity of the isolated recombinant B3/4 editing domain from PheRS, overview | Thermus thermophilus | ? | - |
? | |
additional information | editing activity of the isolated recombinant B3/4 editing domain from PheRS, overview | Escherichia coli | ? | - |
? | |
additional information | editing activity of the isolated recombinant B3/4 editing domain from PheRS, overview | Pyrococcus horikoshii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | PheRS belongs to class IIc and is a tetrameric enzyme consisting of two alphabeta heterodimers. The B3/4 domain of the beta-subunit catalyzes the editing, domain architecture, overview | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
Phenylalanyl-tRNA synthetase | - |
Thermus thermophilus |
Phenylalanyl-tRNA synthetase | - |
Escherichia coli |
Phenylalanyl-tRNA synthetase | - |
Pyrococcus horikoshii |
PheRS | - |
Thermus thermophilus |
PheRS | - |
Escherichia coli |
PheRS | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus | |
ATP | - |
Escherichia coli | |
ATP | - |
Pyrococcus horikoshii |