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Literature summary for 6.1.1.20 extracted from
- Genetic tools for allelic replacement in Burkholderia species (2008), Appl. Environ. Microbiol., 74, 4498-4508.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant pheS, alpha-subunit encoding, gene in different Burkholderia species, e.g. Burkholderia thailandensis, allelic replacement | Burkholderia pseudomallei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | engineering of a mutated counter-selectable marker based on the Burkholderia pseudomallei PheS, i.e. the alpha-subunit of PheRS protein, effectiveness in three different transformed Burkholderia species, the mutant PheS protein effectively killed 100% of the bacteria in the presence of 0.1% 4-chlorophenylalanine, overview. Assembling of mutant pheS on several allelic replacement vectors, in addition to construction of selectable markers based on tellurite and trimethoprim resistance that are excisable by flanking unique FLP recombination target sequences, overview | Burkholderia pseudomallei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Burkholderia pseudomallei |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Burkholderia pseudomallei | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia pseudomallei | - |
gene pheS | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Burkholderia pseudomallei | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Phenylalanyl-tRNA synthetase | - |
Burkholderia pseudomallei |
| PheRS | - |
Burkholderia pseudomallei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Burkholderia pseudomallei | |
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