Protein Variants | Comment | Organism |
---|---|---|
A249G/A356W | mutant enzyme with A294G mutation in alpha-subunit and A356W mutation in beta-subunit, kcat/Km is 6.6% of wild-type value | Escherichia coli |
A249G/E334A | mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit, kcat/Km is 60% of wild-type value | Escherichia coli |
A249G/H265A | mutant enzyme with A294G mutation in alpha-subunit and H265A mutation in beta-subunit, kcat/Km is 52% of wild-type value | Escherichia coli |
A249G/H265L | mutant enzyme with A294G mutation in alpha-subunit and H265L mutation in beta-subunit, kcat/Km is 63% of wild-type value | Escherichia coli |
A249G/T354W | mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit, kcat/Km is 26% of wild-type value | Escherichia coli |
A294G | mutant enzyme with A294G mutation in alpha-subunit, kcat/Km is 58% of wild-type value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
L-Phe | wild-type enzyme | Escherichia coli | |
0.0212 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit | Escherichia coli | |
0.0225 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit | Escherichia coli | |
0.0261 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit | Escherichia coli | |
0.0282 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and A356W mutation in beta-subunit | Escherichia coli | |
0.0312 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and H265L mutation in beta-subunit | Escherichia coli | |
0.0331 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and H265A mutation in beta-subunit | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | phenylalanyl-tRNA synthetase misactivates tyrosine and subsequently corrects such errors through hydrolysis of tyrosyl-adenylate and Tyr-tRNAPhe. Editing by phenylalanyl-tRNA synthetase is essential for faithful translation of the genetic code | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Phe + tRNAPhe | - |
Escherichia coli | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
additional information | phenylalanyl-tRNA synthetase misactivates tyrosine and subsequently corrects such errors through hydrolysis of tyrosyl-adenylate and Tyr-tRNAPhe. Editing by phenylalanyl-tRNA synthetase is essential for faithful translation of the genetic code | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.975 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit | Escherichia coli | |
1.72 | - |
L-Phe | wild-type enzyme | Escherichia coli | |
2.04 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit | Escherichia coli | |
2.645 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit | Escherichia coli | |
2.88 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and H265A mutation in beta-subunit | Escherichia coli | |
3 | 6 | L-Phe | mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit | Escherichia coli | |
3.12 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and A356W mutation in beta-subunit | Escherichia coli | |
3.3 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and H265L mutation in beta-subunit | Escherichia coli | |
6.08 | - |
L-Phe | mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit | Escherichia coli |