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Literature summary for 6.1.1.20 extracted from

  • Datta, D.; Wang, P.; Carrico, I.S.; Mayo, S.L.; Tirrell, D.A.
    A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins (2002), J. Am. Chem. Soc., 124, 5652-5653.
    View publication on PubMed
Search for more literature for 6.1.1.20

Application

Application Comment Organism
biotechnology design of an enzyme variant which incorporates aryl ketones into proteins Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of mutants Escherichia coli

Protein Variants

Protein Variants Comment Organism
A294G mutation of the catalytical alpha-subunit, constructed mutant shows relaxed substrate specificity, efficient incorporation of p-iodo, p-ethynyl-, p-cyano-, and p-azidophenylalanines, but not p-acetylphenylalanine, into protein in the Escherichia coli host Escherichia coli
T251G constructed mutant shows relaxed substrate specificity, efficient incorporation of p-acetylphenylalanine and reactive aryl ketones into protein in the Escherichia coli host Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Escherichia coli
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-acetyl-L-phenylalanine + tRNAPhe recombinant mutant T251G, no activity with A294G Escherichia coli AMP + diphosphate + 4-acetyl-L-phenylalanyl-tRNAPhe
-
 ?
ATP + 4-azido-L-phenylalanine + tRNAPhe recombinant mutant A294G Escherichia coli AMP + diphosphate + 4-azido-L-phenylalanyl-tRNAPhe
-
 ?
ATP + 4-bromo-L-phenylalanine + tRNAPhe recombinant mutant A294G Escherichia coli AMP + diphosphate + 4-bromo-L-phenylalanyl-tRNAPhe
-
 ?
ATP + 4-cyano-L-phenylalanine + tRNAPhe recombinant mutant A294G Escherichia coli AMP + diphosphate + 4-cyano-L-phenylalanyl-tRNAPhe
-
 ?
ATP + 4-ethynyl-L-phenylalanine + tRNAPhe recombinant mutant A294G Escherichia coli AMP + diphosphate + 4-ethynyl-L-phenylalanyl-tRNAPhe
-
 ?
ATP + 4-iodo-L-phenylalanine + tRNAPhe recombinant mutant A294G Escherichia coli AMP + diphosphate + 4-iodo-L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Synonyms

Synonyms Comment Organism
CML33
-
 Escherichia coli
FRS
-
 Escherichia coli
HSPC173
-
 Escherichia coli
L-Phenylalanyl-tRNA synthetase
-
 Escherichia coli
Phenylalanine translase
-
 Escherichia coli
Phenylalanine--tRNA ligase
-
 Escherichia coli
Phenylalanine-tRNA synthetase
-
 Escherichia coli
Phenylalanyl transfer ribonucleic acid synthetase
-
 Escherichia coli
Phenylalanyl-transfer ribonucleate synthetase
-
 Escherichia coli
Phenylalanyl-transfer RNA ligase
-
 Escherichia coli
Phenylalanyl-transfer RNA synthetase
-
 Escherichia coli
Phenylalanyl-tRNA ligase
-
 Escherichia coli
Phenylalanyl-tRNA synthetase
-
 Escherichia coli
PheRS
-
 Escherichia coli
Synthetase, phenylalanyl-transfer ribonucleate
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli