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Literature summary for 6.1.1.20 extracted from
- Prokaryotic and eukaryotic tetrameric phenylalanyl-tRNA synthetases display conservation of the binding mode of the tRNAPhe CCA end (2003), Biochemistry, 42, 10697-10708.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PheOH-AMP | - |
Homo sapiens |  |
| tRNAPhe Cp75 | tRNAPhe with cytosine phosphate residue at position 75 | Homo sapiens | |
| tRNAPhe s4-U75 | tRNAPhe with 4-thiouridine residue at position 75 | Homo sapiens | |
| tRNAPhe s4-U76 | tRNAPhe with 4-thiouridine residue at position 76 | Homo sapiens | |
| tRNAPhe s4-U77 | tRNAPhe with 4-thiouridine residue at position 77 | Homo sapiens | |
| tRNAPhe s4-Up77 | tRNAPhe with 4-thiouridine phosphate residue at position 77 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
L-phenylalanine | 25°C | Homo sapiens | |
| 0.1 | - |
ATP | 25°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Homo sapiens | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe | tRNAPhe substrate binding mechanism, base-specific interactions between the 3'-end of the tRNA and the enzymes alpha- and beta-subunit | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.19 | - |
pure enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CML33 | - |
Homo sapiens |
| FRS | - |
Homo sapiens |
| HSPC173 | - |
Homo sapiens |
| L-Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| Phenylalanine translase | - |
Homo sapiens |
| Phenylalanine--tRNA ligase | - |
Homo sapiens |
| Phenylalanine-tRNA synthetase | - |
Homo sapiens |
| Phenylalanyl transfer ribonucleic acid synthetase | - |
Homo sapiens |
| Phenylalanyl-transfer ribonucleate synthetase | - |
Homo sapiens |
| Phenylalanyl-transfer RNA ligase | - |
Homo sapiens |
| Phenylalanyl-transfer RNA synthetase | - |
Homo sapiens |
| Phenylalanyl-tRNA ligase | - |
Homo sapiens |
| Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| PheRS | - |
Homo sapiens |
| Synthetase, phenylalanyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
PheOH-AMP | 25°C | Homo sapiens | |
| 0.00035 | - |
tRNAPhe s4-Up77 | 25°C | Homo sapiens | |
| 0.0004 | - |
tRNAPhe s4-U77 | 25°C | Homo sapiens | |
| 0.00064 | - |
tRNAPhe s4-U76 | 25°C | Homo sapiens | |
| 0.00069 | - |
tRNAPhe Cp75 | 25°C | Homo sapiens | |
| 0.00071 | - |
tRNAPhe s4-U75 | 25°C | Homo sapiens |
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Release 2023.1 (January 2023)
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