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Literature summary for 6.1.1.20 extracted from
- Human phenylalanyl-tRNA synthetase: cloning, characterization of the deduced amino acid sequences in terms of the structural domains and coordinately regulated expression of the alpha and beta subunits in chronic myeloid leukemia cells (1999), Biochem. Biophys. Res. Commun., 255, 765-773.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination of the alpha- and beta-subunit and analysis, expression in COS-7 cells | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta, amino acid determination | Homo sapiens |
| 55000 | - |
1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta2, amino acid determination | Homo sapiens |
| 57000 | - |
1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta, amino acid determination | Homo sapiens |
| 57000 | - |
1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta2, amino acid determination | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Homo sapiens | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |  |
| additional information | Homo sapiens | the catalytic function resides in the alpha-subunit, while the beta-subunit provides several binding-like domains for OB, RNP, SH3, and DNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9NSD9 | class II enzyme | - |
| Homo sapiens | Q9Y285 | class II enzyme | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe | tRNA recognition and binding mode | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-549 cell | lung carcinoma cell line | Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| embryo | - |
Homo sapiens | - |
| G-361 cell | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| HeLa-S3 cell | high content | Homo sapiens | - |
| HL-60 cell | promyelocytic leukemia cell line, high content | Homo sapiens | - |
| K-562 cell | chronic myelogenous leukemic cell line, high content | Homo sapiens | - |
| kidney | low content, embryonic and adult | Homo sapiens | - |
| liver | low content | Homo sapiens | - |
| lung | low content | Homo sapiens | - |
| MOLT-4 cell | lymphoblastic leukemia cell line | Homo sapiens | - |
| additional information | enzyme content is increased in cancer cell lines, with the beta-subunit showing higher expression levels than the alpha-subunit | Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| placenta | low content | Homo sapiens | - |
| RAJI cell | - |
Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| SW-480 cell | colorectal adenocarcinoma cell line, high content | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
| additional information | the catalytic function resides in the alpha-subunit, while the beta-subunit provides several binding-like domains for OB, RNP, SH3, and DNA | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta, amino acid determination | Homo sapiens |
| dimer | 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta2, amino acid determination | Homo sapiens |
| More | the catalytic function resides in the alpha-subunit, while the beta-subunit provides several binding-like domains for OB, RNP, SH3, and DNA | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CML33 | - |
Homo sapiens |
| FRS | - |
Homo sapiens |
| HSPC173 | - |
Homo sapiens |
| L-Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| Phenylalanine translase | - |
Homo sapiens |
| Phenylalanine--tRNA ligase | - |
Homo sapiens |
| Phenylalanine-tRNA synthetase | - |
Homo sapiens |
| Phenylalanyl transfer ribonucleic acid synthetase | - |
Homo sapiens |
| Phenylalanyl-transfer ribonucleate synthetase | - |
Homo sapiens |
| Phenylalanyl-transfer RNA ligase | - |
Homo sapiens |
| Phenylalanyl-transfer RNA synthetase | - |
Homo sapiens |
| Phenylalanyl-tRNA ligase | - |
Homo sapiens |
| Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| PheRS | - |
Homo sapiens |
| Synthetase, phenylalanyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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