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Literature summary for 6.1.1.2 extracted from
- Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains (2002), J. Biol. Chem., 277, 14343-14349.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00005 | - |
Archeoglobus fulgidus tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00005 | - |
Archeoglobus fulgidus tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00006 | - |
Archeoglobus fulgidus tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00006 | - |
Archeoglobus fulgidus tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00009 | - |
Bacillus subtilis tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.0001 | - |
Bacillus subtilis tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00012 | - |
Bacillus subtilis tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00012 | - |
Bacillus subtilis tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00017 | - |
bovine tRNATrp(G73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00018 | - |
bovine tRNATrp(G73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00021 | - |
bovine tRNATrp(G73A) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.00022 | - |
bovine tRNATrp(G73C) | pH 7.0, 65°C | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | O28579 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73, wild-type) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73, wild-type) | - |
? |  |
| ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73C) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73C) | - |
? | |
| ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73G) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73G) | - |
? | |
| ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73U) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73U) | - |
? | |
| ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73, wild-type) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73, wild-type) | - |
? | |
| ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73C) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73C) | - |
? | |
| ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73G) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73G) | - |
? | |
| ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73U) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73U) | - |
? | |
| ATP + L-tryptophan + bovine tRNATrp(G73, wild-type) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73, wild-type) | - |
? | |
| ATP + L-tryptophan + bovine tRNATrp(G73A) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73A) | - |
? | |
| ATP + L-tryptophan + bovine tRNATrp(G73C) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73C) | - |
? | |
| ATP + L-tryptophan + bovine tRNATrp(G73U) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73U) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpRS | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay at | Archaeoglobus fulgidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
bovine tRNATrp(G73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.04 | - |
bovine tRNATrp(G73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.04 | - |
Bacillus subtilis tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.05 | - |
Bacillus subtilis tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.06 | - |
bovine tRNATrp(G73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.06 | - |
Bacillus subtilis tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.07 | - |
Archeoglobus fulgidus tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.08 | - |
bovine tRNATrp(G73A) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.09 | - |
Archeoglobus fulgidus tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.11 | - |
Archeoglobus fulgidus tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.14 | - |
Bacillus subtilis tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
| 0.53 | - |
Archeoglobus fulgidus tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Archaeoglobus fulgidus |
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