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Literature summary for 6.1.1.2 extracted from

  • Hughes, R.A.; Ellington, A.D.
    Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA (2010), Nucleic Acids Res., 38, 6813-6830.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.2

Application

Application Comment Organism
synthesis a functional, orthogonal suppressor tRNA pair can prove useful for the incorporation of bulky, unnatural amino acids into the genetic code. Role of tRNA flexibility in molecular recognition and the engineering and evolution of tRNA specificity Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information developed an orthogonal tryptophanyl tRNA synthetase and tRNA pair, derived from Saccharomyces cerevisiae, modification of the G:C content of the anticodon stem and therefore reducing the structural flexibility of this stem eliminates misacylation by the Escherichia coli lysyl tRNA synthetase, and led to the development of a functional, orthogonal suppressor pair Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Escherichia coli CSH108
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Synonyms

Synonyms Comment Organism
TrpRS
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 Escherichia coli
tyrosyl tRNA synthetase
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 Escherichia coli

General Information

General Information Comment Organism
physiological function the Escherichia coli lysyl tRNA synthetase is responsible for misacylating the initial amber suppressor version of Saccharomyces cerevisiae tryptophanyl tRNA. Role of tRNA flexibility in molecular recognition and the engineering and evolution of tRNA specificity Escherichia coli