Cloned (Comment) | Organism |
---|---|
the trpS1 gene is inserted into the vector pET28a for expression in Escherichia coli BL21DE3 cells | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
the structure of tryptophanyl-tRNA synthetase in complex with tryptophanyl-5' AMP is solved at 3.0 A resolution | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Pyrococcus horikoshii | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59584 | - |
- |
Purification (Comment) | Organism |
---|---|
on a Ni-affinity column | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Pyrococcus horikoshii | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Pyrococcus horikoshii |
Tryptophanyl-tRNA synthetase | - |
Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |