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Literature summary for 6.1.1.2 extracted from

  • Charriere, F.; Helgadottir, S.; Horn, E.K.; Soell, D.; Schneider, A.
    Dual targeting of a single tRNA(Trp) requires two different tryptophanyl-tRNA synthetases in Trypanosoma brucei (2006), Proc. Natl. Acad. Sci. USA, 103, 6847-6852.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
isozymes TrpRS1 and TrpRS2 encoded by two distinct genes, phylogenetic tree, expression in Escherichia coli Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isozyme TrpRS1 Trypanosoma brucei 5829
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mitochondrion isozyme TrpRS2 contains a mitochondrial targeting signal, two genes exist due to the need for a mitochondrial synthetase with extended substrate specificity to achieve aminoacylation of the imported thiolated and edited tRNATrp Trypanosoma brucei 5739
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additional information most trypanosomal aminoacyl-tRNA synthetases are encoded by single-copy genes, suggesting the use of the same enzyme in the cytosol and mitochondrion Trypanosoma brucei
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
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 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Trypanosoma brucei both TrpRS1 and TrpRS2 are essential for growth and required for cytosolic and mitochondrial tryptophanyl-tRNA formation, respectively, the edited anticodon and the mitochondria-specific thiolation of U33 in the imported tRNATrp act as antideterminants for the cytosolic TrpRS1, the mitochondrion of Trypanosoma brucei does not encode any tRNAs, this deficiency is compensated for by the import of a small fraction of nearly all of its cytosolic tRNAs from the host AMP + diphosphate + L-tryptophanyl-tRNATrp
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 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
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 isozymes TrpRS1 and TrpRS2 encoded by two distinct genes
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Purification (Commentary)

Purification (Comment) Organism
recombinant isozymes TrpRS1 and TrpRS2 from Escherichia coli to over 95% purity Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp both TrpRS1 and TrpRS2 are essential for growth and required for cytosolic and mitochondrial tryptophanyl-tRNA formation, respectively, the edited anticodon and the mitochondria-specific thiolation of U33 in the imported tRNATrp act as antideterminants for the cytosolic TrpRS1, the mitochondrion of Trypanosoma brucei does not encode any tRNAs, this deficiency is compensated for by the import of a small fraction of nearly all of its cytosolic tRNAs from the host Trypanosoma brucei AMP + diphosphate + L-tryptophanyl-tRNATrp
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 ?
ATP + L-tryptophan + tRNATrp because of editing in the mitochondrion, cytosolic and mitochondrial tRNATrp differ in an anticodon nucleotide and substrate specificity, although neither enzyme is able to recognize in vitro transcripts corresponding to unedited or edited tRNATrp, both efficiently aminoacylate isolated Trypanosoma brucei cytosolic tRNA in in vitro charging assays, overview Trypanosoma brucei AMP + diphosphate + L-tryptophanyl-tRNATrp
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 ?

Synonyms

Synonyms Comment Organism
TrpRS
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 Trypanosoma brucei
Tryptophanyl-tRNA synthetase
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 Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma brucei