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Literature summary for 6.1.1.2 extracted from

  • Kapustina, M.; Carter, C.W.
    Computational studies of tryptophanyl-tRNA synthetase: activation of ATP by induced-fit (2006), J. Mol. Biol., 362, 1159-1180.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information multiple ligand binding conformation simulations with a virtual polyA mobile loop mutant and a virtual K111A mutant, interaction analysis, overview Geobacillus stearothermophilus

General Stability

General Stability Organism
ligand-linked conformational stability changes associated with this catalytic cycle, overview Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+-ATP interactions are coupled to interactions between ATP and active-site lysine side-chains Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Geobacillus stearothermophilus
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AMP + diphosphate + L-tryptophanyl-tRNATrp
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?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P00953
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp
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Geobacillus stearothermophilus AMP + diphosphate + L-tryptophanyl-tRNATrp
-
?
ATP + L-tryptophan + tRNATrp a two-step reaction of amino acid activation followed by aminoacylation, catalysis of amino acid activation involves three allosteric states: 1.open, 2. closed pre-transition state involving Mg2+ and an active site lysine residue, and 3. closed producs, the interconversions of these states entail significant domain motions driven by ligand binding, molecular dynamic simulations, overview Geobacillus stearothermophilus AMP + diphosphate + L-tryptophanyl-tRNATrp
-
?
additional information ligand-linked conformational stability changes associated with this catalytic cycle, overview Geobacillus stearothermophilus ?
-
?

Subunits

Subunits Comment Organism
More open and closed enzyme conformations, overview Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
TrpRS
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Geobacillus stearothermophilus
Tryptophanyl-tRNA synthetase
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Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
ATP Mg2+-ATP interactions are coupled to interactions between ATP and active-site lysine side-chains Geobacillus stearothermophilus