Protein Variants | Comment | Organism |
---|---|---|
additional information | multiple ligand binding conformation simulations with a virtual polyA mobile loop mutant and a virtual K111A mutant, interaction analysis, overview | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
ligand-linked conformational stability changes associated with this catalytic cycle, overview | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+-ATP interactions are coupled to interactions between ATP and active-site lysine side-chains | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Geobacillus stearothermophilus | - |
AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P00953 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | a two-step reaction of amino acid activation followed by aminoacylation, catalysis of amino acid activation involves three allosteric states: 1.open, 2. closed pre-transition state involving Mg2+ and an active site lysine residue, and 3. closed producs, the interconversions of these states entail significant domain motions driven by ligand binding, molecular dynamic simulations, overview | Geobacillus stearothermophilus | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
additional information | ligand-linked conformational stability changes associated with this catalytic cycle, overview | Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | open and closed enzyme conformations, overview | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Geobacillus stearothermophilus |
Tryptophanyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | Mg2+-ATP interactions are coupled to interactions between ATP and active-site lysine side-chains | Geobacillus stearothermophilus |