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Literature summary for 6.1.1.2 extracted from

  • Jia, J.; Chen, X.L.; Guo, L.T.; Yu, Y.D.; Ding, J.P.; Jin, Y.X.
    Residues Lys-149 and Glu-153 switch the aminoacylation of tRNA(Trp) in Bacillus subtilis (2004), J. Biol. Chem., 279, 41960-41965.
    View publication on PubMed
Search for more literature for 6.1.1.2

Protein Variants

Protein Variants Comment Organism
E153D ratio of turnover number to Km-value for ATP is 109% of wild-type value, ratio of turnover number to Km-value for Trp is 114% of wild-type value, ratio of turnover number to Km-value for Bacillus subtilis tRNATrp is 89% of wild-type value Bacillus subtilis
E153G no detectable activity in activation of Trp unless tRNATRp is added to the reaction Bacillus subtilis
E153K ratio of turnover number to Km-value for ATP is 79% of wild-type value, ratio of turnover number to Km-value for Trp is 68% of wild-type value, ratio of turnover number to Km-value for Bacillus subtilis tRNATrp is 3% of wild-type value Bacillus subtilis
K149D/E153R ratio of turnover number to Km-value for ATP is 30% of wild-type value, ratio of turnover number to Km-value for Trp is 10% of wild-type value, ratio of turnover number to Km-value for Bacillus subtilis tRNATrp is 1% of wild-type value Bacillus subtilis
K149E ratio of turnover number to Km-value for ATP is 99.7% of wild-type value, ratio of turnover number to Km-value for Trp is 89% of wild-type value, ratio of turnover number to Km-value for Bacillus subtilis tRNATrp is 37% of wild-type value Bacillus subtilis
K149G ratio of turnover number to Km-value for ATP is 111% of wild-type value, ratio of turnover number to Km-value for Trp is 88% of wild-type value, ratio of turnover number to Km-value for Bacillus subtilis tRNATrp is 55% of wild-type value Bacillus subtilis
K149R ratio of turnover number to Km-value for ATP is 112% of wild-type value, ratio of turnover number to Km-value for Trp is 98% of wild-type value, ratio of turnover number to Km-value for Bacillus subtilis tRNATrp is 64% of wild-type value Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149R, aminoacylation Bacillus subtilis
0.00061
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, wild-type enzyme, aminoacylation Bacillus subtilis
0.00087
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149E, aminoacylation Bacillus subtilis
0.00088
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme E153D, aminoacylation Bacillus subtilis
0.00113
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme E153K, aminoacylation Bacillus subtilis
0.00121
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149G, aminoacylation Bacillus subtilis
0.00123
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme E153G, aminoacylation Bacillus subtilis
0.00126
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149D/E153R, aminoacylation Bacillus subtilis
0.0033
-
 Trp 25°C, pH 9.0, mutant enzyme E153D, activation of Trp Bacillus subtilis
0.0037
-
 Trp 25°C, pH 9.0, mutant enzyme K149E, activation of Trp Bacillus subtilis
0.0038
-
 Trp 25°C, pH 9.0, mutant enzyme K149G, activation of Trp Bacillus subtilis
0.0046
-
 Trp 25°C, pH 9.0, wild-type enzyme, activation of Trp Bacillus subtilis
0.0099
-
 Trp 25°C, pH 9.0, mutant enzyme K149R, activation of Trp Bacillus subtilis
0.041
-
 ATP 25°C, pH 9.0, mutant enzyme K149G, activation of Trp Bacillus subtilis
0.046
-
 ATP 25°C, pH 9.0, mutant enzyme K149E, activation of Trp Bacillus subtilis
0.048
-
 ATP 25°C, pH 9.0, mutant enzyme E153D, activation of Trp Bacillus subtilis
0.06
-
 Trp 25°C, pH 9.0, mutant enzyme K149D/E153R, activation of Trp Bacillus subtilis
0.065
-
 ATP 25°C, pH 9.0, wild-type enzyme, activation of Trp Bacillus subtilis
0.121
-
 ATP 25°C, pH 9.0, mutant enzyme K149R, activation of Trp Bacillus subtilis
0.128
-
 Trp 25°C, pH 9.0, mutant enzyme E153K, activation of Trp Bacillus subtilis
0.278
-
 ATP 25°C, pH 9.0, mutant enzyme K149D/E153R, activation of Trp Bacillus subtilis
1.552
-
 ATP 25°C, pH 9.0, mutant enzyme E153K, activation of Trp Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp
-
 Bacillus subtilis AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?

Synonyms

Synonyms Comment Organism
TrpRS
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149D/E153R, aminoacylation Bacillus subtilis
0.02
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme E153K, aminoacylation Bacillus subtilis
0.03
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme E153D, aminoacylation Bacillus subtilis
0.32
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149R, 0.32 aminoacylation Bacillus subtilis
0.32
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, wild-type enzyme, aminoacylation Bacillus subtilis
0.67
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149E, aminoacylation Bacillus subtilis
0.78
-
 tRNATrp 22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149G, aminoacylation Bacillus subtilis
2 8 Trp 25°C, pH 9.0, mutant enzyme K149D/E153R, activation of Trp Bacillus subtilis
15
-
 ATP 25°C, pH 9.0, mutant enzyme K149G, activation of Trp Bacillus subtilis
15
-
 Trp 25°C, pH 9.0, mutant enzyme K149G, activation of Trp Bacillus subtilis
16
-
 ATP 25°C, pH 9.0, mutant enzyme K149E, activation of Trp Bacillus subtilis
16
-
 Trp 25°C, pH 9.0, mutant enzyme K149E, activation of Trp Bacillus subtilis
17
-
 ATP 25°C, pH 9.0, mutant enzyme E153D, activation of Trp Bacillus subtilis
18
-
 Trp 25°C, pH 9.0, mutant enzyme E153D, activation of Trp Bacillus subtilis
22
-
 ATP 25°C, pH 9.0, wild-type enzyme, activation of Trp Bacillus subtilis
22
-
 Trp 25°C, pH 9.0, wild-type enzyme, activation of Trp Bacillus subtilis
27
-
 ATP 25°C, pH 9.0, mutant enzyme K149D/E153R, activation of Trp Bacillus subtilis
46
-
 ATP 25°C, pH 9.0, mutant enzyme K149R, activation of Trp Bacillus subtilis
46
-
 Trp 25°C, pH 9.0, mutant enzyme K149R, activation of Trp Bacillus subtilis
419
-
 ATP 25°C, pH 9.0, mutant enzyme E153K, activation of Trp Bacillus subtilis
419
-
 Trp 25°C, pH 9.0, mutant enzyme E153K, activation of Trp Bacillus subtilis