Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Codon Plus RP cells | Canavalia ensiformis |
expressed in Escherichia coli BL21 Codon Plus RP cells | Glycine max |
expressed in Escherichia coli BL21 Codon Plus RP cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
0.0014 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis | |
0.0017 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
0.0022 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
0.0024 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
0.0041 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canavalia ensiformis | B2G3G6 | - |
- |
Escherichia coli | B1XHE4 | - |
- |
Glycine max | B6ETP1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Source 15Q column chromatography | Glycine max |
Ni-NTA column chromatography and Source15Q column chromatography | Canavalia ensiformis |
Ni-NTA column chromatography and Source15Q column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg(ACG) | tRNA from Escherichia coli | Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg(ACG) | - |
? | |
ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg(ACG) | - |
? | |
ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Escherichia coli | AMP + diphosphate + L-arginyl-soybean tRNAArg(ACG) | - |
? | |
ATP + L-arginine + tRNAArg(ACG) | tRNA from Escherichia coli | Glycine max | AMP + diphosphate + L-arginyl-tRNAArg(ACG) | - |
? | |
ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Glycine max | AMP + diphosphate + L-arginyl-tRNAArg(ACG) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Arginyl-tRNA synthetase | - |
Canavalia ensiformis |
Arginyl-tRNA synthetase | - |
Glycine max |
Arginyl-tRNA synthetase | - |
Escherichia coli |
ArgRS | - |
Canavalia ensiformis |
ArgRS | - |
Glycine max |
ArgRS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
0.02 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli | |
0.03 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
0.04 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
0.055 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
0.105 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Canavalia ensiformis | |
ATP | - |
Glycine max | |
ATP | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli | |
5.5 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
14 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
22.7 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
25.2 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
75 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis |