Cloned (Comment) | Organism |
---|---|
into the TOPO vector and subsequently into the vector pET28c for expression in Escherichia coli BL21DE3codon+ cells | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
a crystal structure of a complex of Arg-tRNA synthetase, tRNAArgCCU, and the ATP analog ANP is determined to 2.0 A resolution | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
DELTANArgRS | lacking the N-terminal side from the 91st residue | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
tRNAArg | tRNAArgCCU, wild-type ArgRS | Pyrococcus horikoshii | |
0.0038 | - |
tRNAArg | tRNAArgCCU, mutant DELTANArgRS | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | Pyrococcus horikoshii | - |
AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59147 | - |
- |
Purification (Comment) | Organism |
---|---|
on a Ni2+-nitrilotriacetic superflow, a Resource Q, a Hitrap heparin, and a hydroxyapatite column | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Pyrococcus horikoshii | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Arg-tRNA synthetase | - |
Pyrococcus horikoshii |
ArgRS | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
aminoacylaion assay | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aminoacylaion assay | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |