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Literature summary for 6.1.1.19 extracted from

  • Konno, M.; Sumida, T.; Uchikawa, E.; Mori, Y.; Yanagisawa, T.; Sekine, S.; Yokoyama, S.; Yokoyama, S.
    Modeling of tRNA-assisted mechanism of Arg activation based on a structure of Arg-tRNA synthetase, tRNA, and an ATP analog (ANP) (2009), FEBS J., 276, 4763-4779.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the TOPO vector and subsequently into the vector pET28c for expression in Escherichia coli BL21DE3codon+ cells Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
a crystal structure of a complex of Arg-tRNA synthetase, tRNAArgCCU, and the ATP analog ANP is determined to 2.0 A resolution Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
DELTANArgRS lacking the N-terminal side from the 91st residue Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
tRNAArg tRNAArgCCU, wild-type ArgRS Pyrococcus horikoshii
0.0038
-
tRNAArg tRNAArgCCU, mutant DELTANArgRS Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-arginine + tRNAArg Pyrococcus horikoshii
-
AMP + diphosphate + L-arginyl-tRNAArg
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O59147
-
-

Purification (Commentary)

Purification (Comment) Organism
on a Ni2+-nitrilotriacetic superflow, a Resource Q, a Hitrap heparin, and a hydroxyapatite column Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
Pyrococcus horikoshii AMP + diphosphate + L-arginyl-tRNAArg
-
?

Synonyms

Synonyms Comment Organism
Arg-tRNA synthetase
-
Pyrococcus horikoshii
ArgRS
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
aminoacylaion assay Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
aminoacylaion assay Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
ATP
-
Pyrococcus horikoshii