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Literature summary for 6.1.1.19 extracted from
- Two forms of human cytoplasmic arginyl-tRNA synthetase produced from two translation initiations by a single mRNA (2006), Biochemistry, 45, 1338-1344.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTANhcArgRS | N-terminal 72-amino acid deletion mutant with higher specific activity than the wild type enzyme | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
SDS-PAGE, mutant enzyme DELTANhcArgRS | Homo sapiens |
| 75000 | - |
SDS-PAGE, wild type enzyme | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA Superflow resin chromatography and dialysis against 20 mM potassium phosphate buffer (pH 7.5) | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryonic fibroblast | - |
Homo sapiens | - |
| HEK-293 cell | - |
Homo sapiens | - |
| Hep-G2 cell | - |
Homo sapiens | - |
| hepatoma cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.286 | - |
wild type enzyme, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens |
| 0.715 | - |
mutant enzyme DELTANhcArgRS, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine + tRNAArg | - |
Homo sapiens | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arginyltRNA synthetase | - |
Homo sapiens |
| ArgRS | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
tRNAArg | wild type enzyme, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens | |
| 0.8 | - |
tRNAArg | mutant enzyme DELTANhcArgRS, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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