Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | biosynthetic preparation of an enzyme where Trp are replaced by 4-fluorotryptophane | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
tRNAArg | aminoacylation reaction | Escherichia coli | |
0.003 | - |
tRNAArg | aminoacylation reaction, 4-fluorotryptophan-labeled enzyme | Escherichia coli | |
0.012 | - |
L-arginine | aminoacylation reaction | Escherichia coli | |
0.0137 | - |
L-arginine | aminoacylation reaction, 4-fluorotryptophan-labeled enzyme | Escherichia coli | |
0.9 | - |
ATP | aminoacylation reaction | Escherichia coli | |
1.35 | - |
ATP | aminoacylation reaction, 4-fluorotryptophan-labeled enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
incorporation of 4-fluorotryptophan has little effect on activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Escherichia coli | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |