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Literature summary for 6.1.1.19 extracted from

  • Charlier, J.; Gerlo, E.
    Arginyl-tRNA synthetase from Escherichia coli K12. Purification, properties, and sequence of substrate addition (1979), Biochemistry, 18, 3171-3178.
    View publication on PubMed
Search for more literature for 6.1.1.19

General Stability

General Stability Organism
affected by repeated freeze-thaw cycles Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
8-azido-ATP
-
 Escherichia coli
homoarginine
-
 Escherichia coli
p-hydroxymercuribenzoate tRNA or DTT protects against heat inactivation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 60000, SDS-PAGE Escherichia coli
63000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + L-arginine + tRNAArg = AMP + diphosphate + L-arginyl-tRNAArg random addition of substrates with all steps in rapid equilibrium except for the interconversion of the central quarternary complexes Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.984
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 50% v/v glycerol, stable for at least 6 months Escherichia coli

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1 8.5
-
 Escherichia coli