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Literature summary for 6.1.1.18 extracted from

  • Grant, T.D.; Luft, J.R.; Wolfley, J.R.; Snell, M.E.; Tsuruta, H.; Corretore, S.; Quartley, E.; Phizicky, E.M.; Grayhack, E.J.; Snell, E.H.
    The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA (2013), J. Mol. Biol., 425, 2480-2493.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain BCY123 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
microbatch-under-oil method, using 50 mM NH4Br, 50 mM KC2H3O2, 100 mM HEPES (pH 7.5), and 20% (w/v) polyethylene glycol 20000 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89100
-
x * 89100, small-angle X-ray scattering Saccharomyces cerevisiae
93100
-
x * 93100, calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamine + tRNAGln Saccharomyces cerevisiae
-
AMP + diphosphate + L-glutaminyl-tRNAGln
-
?
ATP + L-glutamine + tRNAGln Saccharomyces cerevisiae ATCC 204508
-
AMP + diphosphate + L-glutaminyl-tRNAGln
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P13188
-
-
Saccharomyces cerevisiae ATCC 204508 P13188
-
-

Purification (Commentary)

Purification (Comment) Organism
IgG Sepharose column chromatography and Superdex HiLoad 16/60 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamine + tRNAGln
-
Saccharomyces cerevisiae AMP + diphosphate + L-glutaminyl-tRNAGln
-
?
ATP + L-glutamine + tRNAGln
-
Saccharomyces cerevisiae ATCC 204508 AMP + diphosphate + L-glutaminyl-tRNAGln
-
?

Subunits

Subunits Comment Organism
? x * 89100, small-angle X-ray scattering Saccharomyces cerevisiae
? x * 93100, calculated from amino acid sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Gln4
-
Saccharomyces cerevisiae
GlnRS
-
Saccharomyces cerevisiae
Glutaminyl-tRNA synthetase
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae