Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain BCY123 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
microbatch-under-oil method, using 50 mM NH4Br, 50 mM KC2H3O2, 100 mM HEPES (pH 7.5), and 20% (w/v) polyethylene glycol 20000 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89100 | - |
x * 89100, small-angle X-ray scattering | Saccharomyces cerevisiae |
93100 | - |
x * 93100, calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + tRNAGln | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
ATP + L-glutamine + tRNAGln | Saccharomyces cerevisiae ATCC 204508 | - |
AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P13188 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | P13188 | - |
- |
Purification (Comment) | Organism |
---|---|
IgG Sepharose column chromatography and Superdex HiLoad 16/60 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + tRNAGln | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
ATP + L-glutamine + tRNAGln | - |
Saccharomyces cerevisiae ATCC 204508 | AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 89100, small-angle X-ray scattering | Saccharomyces cerevisiae |
? | x * 93100, calculated from amino acid sequence | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Gln4 | - |
Saccharomyces cerevisiae |
GlnRS | - |
Saccharomyces cerevisiae |
Glutaminyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |