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Literature summary for 6.1.1.17 extracted from

  • O'Donoghue, P.; Sheppard, K.; Nureki, O.; Soell, D.
    Rational design of an evolutionary precursor of glutaminyl-tRNA synthetase (2011), Proc. Natl. Acad. Sci. USA, 108, 20485-20490.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00133
-
 tRNAGln(CUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
0.00361
-
 tRNAGlu wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
0.00539
-
 tRNAGln(UUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + tRNAGln(CUG) Methanothermobacter thermautotrophicus the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) AMP + diphosphate + L-glutamyl-tRNAGln(CUG)
-
 ?
ATP + L-glutamate + tRNAGln(UUG) Methanothermobacter thermautotrophicus the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) AMP + diphosphate + L-glutamyl-tRNAGln(UUG)
-
 ?
ATP + L-glutamate + tRNAGlu Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGln(CUG) the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGln(CUG)
-
 ?
ATP + L-glutamate + tRNAGln(UUG) the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGln(UUG)
-
 ?
ATP + L-glutamate + tRNAGlu
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Synonyms

Synonyms Comment Organism
GluRS
-
 Methanothermobacter thermautotrophicus
Glutamyl-tRNA synthetase
-
 Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 tRNAGln(UUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
0.04
-
 tRNAGlu wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
0.041
-
 tRNAGln(CUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanothermobacter thermautotrophicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 tRNAGln(UUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
10
-
 tRNAGlu wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
310
-
 tRNAGln(CUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus