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Literature summary for 6.1.1.17 extracted from

  • Mailu, B.M.; Ramasamay, G.; Mudeppa, D.G.; Li, L.; Lindner, S.E.; Peterson, M.J.; DeRocher, A.E.; Kappe, S.H.; Rathod, P.K.; Gardner, M.J.
    A nondiscriminating glutamyl-tRNA synthetase in the Plasmodium apicoplast: the first enzyme in an indirect aminoacylation pathway (2013), J. Biol. Chem., 288, 32539-32552.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Diphosphatase the addition of diphosphatase dramatically stimulates the reaction rate Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli KRX cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
5'-O-[N-(Lglutamyl)sulfamoyl]adenosine competitive inhibitor with respect to L-glutamate Plasmodium falciparum
diphosphate
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000032
-
tRNAGlu in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
0.0000497
-
tRNAGlu in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
0.0000767
-
tRNAGln in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
0.0000911
-
tRNAGln in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
0.001401
-
L-glutamate in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
0.00235
-
ATP in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
apicoplast
-
Plasmodium falciparum 20011
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
x * 63000, SDS-PAGE Plasmodium falciparum
68400
-
x * 68400, calculated from amino acid sequence Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + tRNAGln Plasmodium falciparum
-
AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGlu Plasmodium falciparum
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGln
-
Plasmodium falciparum AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGlu
-
Plasmodium falciparum AMP + diphosphate + L-glutamyl-tRNAGlu
-
?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Plasmodium falciparum
? x * 68400, calculated from amino acid sequence Plasmodium falciparum

Synonyms

Synonyms Comment Organism
GluRS
-
Plasmodium falciparum
Glutamyl-tRNA synthetase
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
tRNAGln in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
0.43
-
tRNAGlu in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
1.18
-
ATP in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
1.39
-
L-glutamate in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
2.11
-
tRNAGln in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
3.61
-
tRNAGlu in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0007
-
5'-O-[N-(Lglutamyl)sulfamoyl]adenosine in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
704
-
ATP in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
990
-
L-glutamate in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
5430
-
tRNAGln in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
8650
-
tRNAGlu in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
23170
-
tRNAGln in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum
112700
-
tRNAGlu in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C Plasmodium falciparum