Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Diphosphatase | the addition of diphosphatase dramatically stimulates the reaction rate | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli KRX cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-[N-(Lglutamyl)sulfamoyl]adenosine | competitive inhibitor with respect to L-glutamate | Plasmodium falciparum | |
diphosphate | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000032 | - |
tRNAGlu | in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.0000497 | - |
tRNAGlu | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.0000767 | - |
tRNAGln | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.0000911 | - |
tRNAGln | in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.001401 | - |
L-glutamate | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.00235 | - |
ATP | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | - |
Plasmodium falciparum | 20011 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 63000, SDS-PAGE | Plasmodium falciparum |
68400 | - |
x * 68400, calculated from amino acid sequence | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | Plasmodium falciparum | - |
AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | Plasmodium falciparum | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | - |
Plasmodium falciparum | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Plasmodium falciparum | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, SDS-PAGE | Plasmodium falciparum |
? | x * 68400, calculated from amino acid sequence | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
GluRS | - |
Plasmodium falciparum |
Glutamyl-tRNA synthetase | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
tRNAGln | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.43 | - |
tRNAGlu | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
1.18 | - |
ATP | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
1.39 | - |
L-glutamate | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
2.11 | - |
tRNAGln | in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
3.61 | - |
tRNAGlu | in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
5'-O-[N-(Lglutamyl)sulfamoyl]adenosine | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
704 | - |
ATP | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
990 | - |
L-glutamate | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
5430 | - |
tRNAGln | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
8650 | - |
tRNAGlu | in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
23170 | - |
tRNAGln | in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
112700 | - |
tRNAGlu | in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C | Plasmodium falciparum |