Literature summary for 6.1.1.17 extracted from

Madore, E.; Florentz, C.; Giege, R.; Sekine, S.; Yokoyama, S.; Lapointe, J.
Effect of modified nucleotides on Escherichia coli tRNAGlu structure and on its aminoacylation by glutamyl-tRNA synthetase. Predominant and distinct roles of the mnm5 and s2 modifications of U34 (1999), Eur. J. Biochem., 266, 1128-1135.

Search for more literature for 6.1.1.17

Protein Variants

Protein Variants	Comment	Organism
additional information	construction and overexpression of tRNAGlu variants, the enzyme shows altered activity with the tRNA mutants, structural characterization	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics, diverse tRNAGlu mutants	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	Escherichia coli	-	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	pure recombinant enzyme from overproducing strain DH5alpha	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + L-glutamate + tRNAGlu = AMP + diphosphate + L-glutamyl-tRNAGlu	the major recognition element of the tRNAGlu is U at position 34	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	-	Escherichia coli	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?
ATP + L-glutamate + tRNAGlu	the major recognition element of the tRNAGlu is U at position 34, activity with wild-type and mutant tRNAs, overview	Escherichia coli	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?

Synonyms

Synonyms	Comment	Organism
GluRS	-	Escherichia coli
Glutamyl-tRNA synthetase	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	diverse tRNAGlu mutants	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)