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Literature summary for 6.1.1.17 extracted from
- Major identity element of glutamine tRNAs from Bacillus subtilis and Escherichia coli in the reaction with B. subtilis glutamyl-tRNA synthetase (1998), Mol. Cells, 8, 459-465.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction and expression in Escherichia coli of mutant forms of Escherichia coli tRNAGln2 and tRNAGln1 and of Bacillus subtilis tRNAGln | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + tRNAGlu | Bacillus subtilis | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
glutamate and glutamine acceptor activity with wild-type and mutant tRNAGlns with native and recombinant enzyme, overview | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + tRNAGln | enzyme is active with the tRNAGln from Bacillus subtilis and the isoacceptor tRNAGln1, but not tRNAGln2, from Escherichia coli, the major recognition element is U at position 34 | Bacillus subtilis | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
| ATP + L-glutamate + tRNAGlu | - |
Bacillus subtilis | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | enzyme utilizes tRNAGlu from Bacillus subtilis, not from Escherichia coli | Bacillus subtilis | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| additional information | glutamate and glutamine acceptor activity with wild-type and mutant tRNAGlns with native and recombinant enzyme, overview | Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GluRS | - |
Bacillus subtilis |
| Glutamyl-tRNA synthetase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus subtilis | |
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Release 2023.1 (January 2023)
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