General Stability | Organism |
---|---|
no stabilization by various sulfydryl reagents, glycerol and glutamic acid | Vigna radiata var. radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
erythro-4-Hydroxy-DL-glutamic acid | glutamate transfer to tRNA | Vigna radiata var. radiata | |
erythro-gamma-methyl-L-glutamic acid | glutamate transfer to tRNA | Vigna radiata var. radiata | |
KCl | 100 mM, 15% inhibition of ATP-diphosphate exchange, 500 mM, 62% inhibition | Vigna radiata var. radiata | |
LiCl | 100 mM, 22% inhibition of ATP-diphosphate exchange, 500 mM, 73% inhibition | Vigna radiata var. radiata | |
NaCl | 100 mM, 40% inhibition of ATP-diphosphate exchange, 500 mM, 91% inhibition | Vigna radiata var. radiata | |
NH4Cl | 100 mM, 31% inhibition of ATP-diphosphate exchange, 500 mM, 82% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
threo-4-Hydroxy-L-glutamic acid | glutamate transfer to tRNA | Caesalpinia bondue | |
threo-4-Hydroxy-L-glutamic acid | glutamate transfer to tRNA | Vigna radiata var. radiata | |
threo-4-Methyl-D-glutamic acid | glutamate transfer to tRNA | Caesalpinia bondue | |
threo-4-Methyl-D-glutamic acid | glutamate transfer to tRNA | Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Vigna radiata var. radiata | |
additional information | - |
additional information | - |
Hemerocallis fulva | |
additional information | - |
additional information | - |
Caesalpinia bondue |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Vigna radiata var. radiata | |
Mg2+ | required | Hemerocallis fulva | |
Mg2+ | required | Caesalpinia bondue | |
Mg2+ | Km: 3.4 mM, ATP-diphosphate exchange | Hemerocallis fulva | |
Mg2+ | Km: 2.2 mM, ATP-diphosphate exchange, 6.3 mM, glutamate-tRNA formation | Caesalpinia bondue | |
Mg2+ | Km: 1.8 mM, ATP-diphosphate exchange, 4.8 mM, glutamate-tRNA formation | Vigna radiata var. radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caesalpinia bondue | - |
- |
- |
Hemerocallis fulva | - |
- |
- |
Vigna radiata var. radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vigna radiata var. radiata |
- |
Hemerocallis fulva |
- |
Caesalpinia bondue |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Hemerocallis fulva | - |
seed | - |
Vigna radiata var. radiata | - |
seed | - |
Caesalpinia bondue | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0148 | - |
- |
Caesalpinia bondue |
0.0263 | - |
- |
Vigna radiata var. radiata |
0.0396 | - |
- |
Hemerocallis fulva |
Storage Stability | Organism |
---|---|
85% loss of activity after 24 h | Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | - |
Vigna radiata var. radiata | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Hemerocallis fulva | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Caesalpinia bondue | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
additional information | ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
additional information | threo-4-methyl-DL-glutamic acid or threo-4-hydroxy-L-glutamic acid can promote ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
additional information | threo-4-methyl-DL-glutamic acid or threo-4-hydroxy-L-glutamic acid can promote ATP-diphosphate exchange | Caesalpinia bondue | ? | - |
? | |
additional information | erythro-4-methyl-L-glutamic acid, erythro-4-hydroxy-DL-glutamic acid, or 2(S),4(S)-4-hydroxy-4-methyl-L-glutamic acid can promote ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
additional information | erythro-4-methyl-L-glutamic acid, erythro-4-hydroxy-DL-glutamic acid, or 2(S),4(S)-4-hydroxy-4-methyl-L-glutamic acid can promote ATP-diphosphate exchange | Hemerocallis fulva | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
12 min assay | Vigna radiata var. radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | ATP-diphosphate exchange | Vigna radiata var. radiata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | 7: rapid drop of activity below, 7.8-8.2: maximal activity | Vigna radiata var. radiata |