Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Methanosarcina mazei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
tRNA1Cys | value above, substrate tRNA1Cys isoacceptor 1 | Methanosarcina mazei | |
0.09 | - |
tRNA1CysA33U | value above, substrate tRNA1Cys isoacceptor 1, containing a A33U mutation | Methanosarcina mazei | |
0.09 | - |
tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A | value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation | Methanosarcina mazei | |
0.11 | - |
tRNA3CysC20U/U21C/A44U/C46A/A47G | value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation | Methanosarcina mazei | |
0.12 | - |
tRNA3CysG57A | value above, substrate tRNA3Cys isoacceptor 3, containing a G57A mutation | Methanosarcina mazei | |
0.13 | - |
tRNA3CysU33A | value above, substrate tRNA3Cys isoacceptor 3, containing a U33A mutation | Methanosarcina mazei | |
0.14 | - |
tRNA3Cys | value above, substrate tRNA3Cys isoacceptor 3 | Methanosarcina mazei | |
0.16 | - |
tRNA2Cys | value above, substrate tRNA2Cys isoacceptor 2 | Methanosarcina mazei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42320 | - |
predicted from cDNA | Methanosarcina mazei |
42324 | - |
gel filtration, 2 * 42324 | Methanosarcina mazei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatographay | Methanosarcina mazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNA1Cys | substrate tRNA1Cys isoacceptor 1 | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA1Cys | - |
? | |
ATP + L-cysteine + tRNA1CysA33U | substrate tRNA1CysA33U isoacceptor 1, containing a A33U mutation | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA1CysA33U | - |
? | |
ATP + L-cysteine + tRNA2Cys | substrate tRNA2Cys isoacceptor 2 | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA2Cys | - |
? | |
ATP + L-cysteine + tRNA3Cys | substrate tRNA3Cys isoacceptor 3 | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA3Cys | - |
? | |
ATP + L-cysteine + tRNA3CysC20U/U21C/A44U/C46A/A47G | substrate tRNA3CysC20U/U21C/A44U/C46A/A47G isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA3CysC20U/U21C/A44U/C46A/A47G | - |
? | |
ATP + L-cysteine + tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A | substrate tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A | - |
? | |
ATP + L-cysteine + tRNA3CysG57A | substrate tRNA3CysG57A isoacceptor 3, containing a G57A mutation | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA3CysG57A | - |
? | |
ATP + L-cysteine + tRNA3CysU33A | substrate tRNA3CysU33A isoacceptor 3, containing a U33A mutation | Methanosarcina mazei | AMP + diphosphate + L-cysteinyl-tRNA3CysU33A | - |
? | |
additional information | in Methanosarcina mazei Cys-tRNACys is synthesized through a canonical cysteinyl-tRNA synthetase (CysRS) as well as via phosphoseryl-tRNACys synthesized by phosphoseryl-tRNA synthetase and this misacylated intermediate is then converted to Cys-tRNACys by Sep-tRNA:Cys-tRNA synthase via a pyridoxal phosphate-dependent mechanism. The genome of Methanosarcina mazei also features three distinct tRNACys isoacceptors, further indicating the unusual and complex nature of Cys-tRNACys synthesis in this organism | Methanosarcina mazei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration, 2 * 42324 | Methanosarcina mazei |
Synonyms | Comment | Organism |
---|---|---|
Cysteinyl-tRNA synthetase | - |
Methanosarcina mazei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
tRNA3Cys | value above, substrate tRNA3Cys isoacceptor 3 | Methanosarcina mazei | |
0.05 | - |
tRNA3CysU33A | value above, substrate tRNA3Cys isoacceptor 3, containing a U33A mutation | Methanosarcina mazei | |
0.06 | - |
tRNA2Cys | value above, substrate tRNA2Cys isoacceptor 2 | Methanosarcina mazei | |
0.09 | - |
tRNA3CysG57A | value above, substrate tRNA3Cys isoacceptor 3, containing a G57A mutation | Methanosarcina mazei | |
0.14 | - |
tRNA3CysC20U/U21C/A44U/C46A/A47G | value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation | Methanosarcina mazei | |
0.16 | - |
tRNA1CysA33U | value above, substrate tRNA1Cys isoacceptor 1, containing a A33U mutation | Methanosarcina mazei | |
0.17 | - |
tRNA1Cys | value above, substrate tRNA1Cys isoacceptor 1 | Methanosarcina mazei | |
0.18 | - |
tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A | value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation | Methanosarcina mazei |