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Literature summary for 6.1.1.16 extracted from

  • Hauenstein, S.I.; Perona, J.J.
    Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei (2008), J. Biol. Chem., 283, 22007-22017.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Methanosarcina mazei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
tRNA1Cys value above, substrate tRNA1Cys isoacceptor 1 Methanosarcina mazei
0.09
-
tRNA1CysA33U value above, substrate tRNA1Cys isoacceptor 1, containing a A33U mutation Methanosarcina mazei
0.09
-
tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation Methanosarcina mazei
0.11
-
tRNA3CysC20U/U21C/A44U/C46A/A47G value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation Methanosarcina mazei
0.12
-
tRNA3CysG57A value above, substrate tRNA3Cys isoacceptor 3, containing a G57A mutation Methanosarcina mazei
0.13
-
tRNA3CysU33A value above, substrate tRNA3Cys isoacceptor 3, containing a U33A mutation Methanosarcina mazei
0.14
-
tRNA3Cys value above, substrate tRNA3Cys isoacceptor 3 Methanosarcina mazei
0.16
-
tRNA2Cys value above, substrate tRNA2Cys isoacceptor 2 Methanosarcina mazei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42320
-
predicted from cDNA Methanosarcina mazei
42324
-
gel filtration, 2 * 42324 Methanosarcina mazei

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatographay Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNA1Cys substrate tRNA1Cys isoacceptor 1 Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA1Cys
-
?
ATP + L-cysteine + tRNA1CysA33U substrate tRNA1CysA33U isoacceptor 1, containing a A33U mutation Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA1CysA33U
-
?
ATP + L-cysteine + tRNA2Cys substrate tRNA2Cys isoacceptor 2 Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA2Cys
-
?
ATP + L-cysteine + tRNA3Cys substrate tRNA3Cys isoacceptor 3 Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA3Cys
-
?
ATP + L-cysteine + tRNA3CysC20U/U21C/A44U/C46A/A47G substrate tRNA3CysC20U/U21C/A44U/C46A/A47G isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA3CysC20U/U21C/A44U/C46A/A47G
-
?
ATP + L-cysteine + tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A substrate tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A
-
?
ATP + L-cysteine + tRNA3CysG57A substrate tRNA3CysG57A isoacceptor 3, containing a G57A mutation Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA3CysG57A
-
?
ATP + L-cysteine + tRNA3CysU33A substrate tRNA3CysU33A isoacceptor 3, containing a U33A mutation Methanosarcina mazei AMP + diphosphate + L-cysteinyl-tRNA3CysU33A
-
?
additional information in Methanosarcina mazei Cys-tRNACys is synthesized through a canonical cysteinyl-tRNA synthetase (CysRS) as well as via phosphoseryl-tRNACys synthesized by phosphoseryl-tRNA synthetase and this misacylated intermediate is then converted to Cys-tRNACys by Sep-tRNA:Cys-tRNA synthase via a pyridoxal phosphate-dependent mechanism. The genome of Methanosarcina mazei also features three distinct tRNACys isoacceptors, further indicating the unusual and complex nature of Cys-tRNACys synthesis in this organism Methanosarcina mazei ?
-
?

Subunits

Subunits Comment Organism
dimer gel filtration, 2 * 42324 Methanosarcina mazei

Synonyms

Synonyms Comment Organism
Cysteinyl-tRNA synthetase
-
Methanosarcina mazei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
tRNA3Cys value above, substrate tRNA3Cys isoacceptor 3 Methanosarcina mazei
0.05
-
tRNA3CysU33A value above, substrate tRNA3Cys isoacceptor 3, containing a U33A mutation Methanosarcina mazei
0.06
-
tRNA2Cys value above, substrate tRNA2Cys isoacceptor 2 Methanosarcina mazei
0.09
-
tRNA3CysG57A value above, substrate tRNA3Cys isoacceptor 3, containing a G57A mutation Methanosarcina mazei
0.14
-
tRNA3CysC20U/U21C/A44U/C46A/A47G value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation Methanosarcina mazei
0.16
-
tRNA1CysA33U value above, substrate tRNA1Cys isoacceptor 1, containing a A33U mutation Methanosarcina mazei
0.17
-
tRNA1Cys value above, substrate tRNA1Cys isoacceptor 1 Methanosarcina mazei
0.18
-
tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation Methanosarcina mazei