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Literature summary for 6.1.1.16 extracted from

  • Ruan, B.; Nakano, H.; Tanaka, M.; Mills, J.A.; DeVito, J.A.; Min, B.; Low, K.B.; Battista, J.R.; Soll, D.
    Cysteinyl-tRNA(Cys) formation in Methanocaldococcus jannaschii: the mechanism is still unknown (2004), J. Bacteriol., 186, 8-14.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.16

Protein Variants

Protein Variants Comment Organism
V27E mutation does not affect the discrimination of the enzyme for serine. 4fold increase in Km-value for cysteine and 9fold reduction of turnover number for ATP Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00064
-
 tRNACys 30°C, pH 7.0, wild-type enzyme Escherichia coli
0.00092
-
 tRNACys 30°C, pH 7.0, mutant enzyme V27E Escherichia coli
0.0072
-
 L-cysteine 30°C, pH 7.0, wild-type enzyme Escherichia coli
0.0282
-
 L-cysteine 30°C, pH 7.0, mutant enzyme V27E Escherichia coli
0.338
-
 ATP 30°C, pH 7.0, wild-type enzyme Escherichia coli
0.412
-
 ATP 30°C, pH 7.0, mutant enzyme V27E Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
 Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
 ATP 30°C, pH 7.0, mutant enzyme V27E Escherichia coli
0.68
-
 tRNACys 30°C, pH 7.0, mutant enzyme V27E Escherichia coli
0.86
-
 L-cysteine 30°C, pH 7.0, mutant enzyme V27E Escherichia coli
2.9
-
 tRNACys 30°C, pH 7.0, wild-type enzyme Escherichia coli
4.4
-
 ATP 30°C, pH 7.0, wild-type enzyme Escherichia coli
4.8
-
 L-cysteine 30°C, pH 7.0, wild-type enzyme Escherichia coli