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Literature summary for 6.1.1.16 extracted from

  • Zhang, C.M.; Christian, T.; Newberry, K.J.; Perona, J.J.; Hou, Y.M.
    Zinc-mediated amino acid discrimination in cysteinyl-tRNA synthetase (2003), J. Mol. Biol., 327, 911-917.
    View publication on PubMed
Search for more literature for 6.1.1.16

Protein Variants

Protein Variants Comment Organism
C28S/C209S site-directed mutagenesis, no ATP-diphosphate exchange activity, residual aminoacylation activity Escherichia coli
C28S/C209S/H234N/E238Q site-directed mutagensis, mutation of all zinc binding ligands, complete loss of bound zinc, weak ability to bind serine, thus loss of amino acid substrate discrimination ability Escherichia coli
C36S/C214S/C244S site-directed mutagenesis, activity is similar to the wild-type enzyme, but the affinity for cysteine binding is increased Escherichia coli
additional information construction of a Co2+-substituted wild-type enzyme, similar properties as the Zn2+-wild-type enzyme but slightly reduced activity Escherichia coli
W205Y site-directed mutagenesis, highly reduced activity, highly increased Km for cysteine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 tRNACys aminoacylation reaction, recombinant Zn2+-wild-type enzyme Escherichia coli
0.0004
-
 tRNACys aminoacylation reaction, recombinant His-tagged Co2+-wild-type enzyme Escherichia coli
0.0012
-
 tRNACys aminoacylation reaction, recombinant His-tagged Zn2+-wild-type enzyme Escherichia coli
0.0066
-
 tRNACys aminoacylation reaction, recombinant W205Y mutant enzyme Escherichia coli
0.022
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme Escherichia coli
0.029
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme Escherichia coli
0.031
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme Escherichia coli
0.095
-
 tRNACys aminoacylation reaction, recombinant C28S/C209S mutant enzyme Escherichia coli
0.22
-
 ATP ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme Escherichia coli
0.25
-
 ATP ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme Escherichia coli
0.29
-
 ATP ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme Escherichia coli
0.77
-
 ATP ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme Escherichia coli
3.61
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute for Zn2+ Escherichia coli
Zn2+ can be substituted by Co2+, tightly bound, 1 ion per enzyme molecule, bound at the base of the active site cleft, primary determinant of selectivity against non-cognate amino acids Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-cysteine + tRNACys Escherichia coli
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
 Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys enzyme is highly specific for L-cysteine without performing an editing reaction, tightly bound zinc is the primary determinant of selectivity against non-cognate amino acids Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Synonyms

Synonyms Comment Organism
CysRS
-
 Escherichia coli
Cysteine translase
-
 Escherichia coli
Cysteine--tRNA ligase
-
 Escherichia coli
Cysteinyl-transfer ribonucleate synthetase
-
 Escherichia coli
Cysteinyl-transfer RNA synthetase
-
 Escherichia coli
Cysteinyl-tRNA synthetase
-
 Escherichia coli
Synthetase, cysteinyl-transfer ribonucleate
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 tRNACys aminoacylation reaction, recombinant C28S/C209S mutant enzyme Escherichia coli
0.007
-
 tRNACys aminoacylation reaction, recombinant W205Y mutant enzyme Escherichia coli
0.28
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme Escherichia coli
0.3
-
 ATP ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme Escherichia coli
0.5
-
 tRNACys aminoacylation reaction, recombinant His-tagged Co2+-wild-type enzyme Escherichia coli
0.9
-
 tRNACys aminoacylation reaction, recombinant Zn2+-wild-type enzyme Escherichia coli
2.5
-
 tRNACys aminoacylation reaction, recombinant His-tagged Zn2+-wild-type enzyme Escherichia coli
35
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme Escherichia coli
57
-
 ATP ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme Escherichia coli
79
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme Escherichia coli
91
-
 ATP ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme Escherichia coli
100
-
 L-cysteine ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme Escherichia coli
142
-
 ATP ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli