Crystallization (Comment) | Organism |
---|---|
purified recombinant free enzyme or cysteine and ATP complexed enzyme, hanging-drop vapour diffusion method, 17°C, 0.001 ml protein solution: 6 mg/ml enzyme, 10 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 mM ATP, 10 mM cysteine, plus equal volume of reservoir solution: 0.1 M sodium cacodylate, pH 6.5, 15-17% PEG 8000, 0.2 M magnesium acetate, 2% tert-butanol, 1-3 weeks, cysteine but not ATP is required for crystal growth, X-ray diffraction structure determination at 2.3-3.0 A resolution, and analysis | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 ion bound at the base of the active site cleft, important for tRNA substrate specificity, single direct interaction with cysteine thiolate substrate | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | Escherichia coli | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P21888 | class I enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-cysteine + tRNACys = AMP + diphosphate + L-cysteinyl-tRNACys | substrate binding mechanism, induced fit | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
ATP + L-cysteine + tRNACys | enzyme is highly specific for L-cysteine and does not possess the editing activity characteristic for other tRNA synthetases | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
CysRS | - |
Escherichia coli |
Cysteine translase | - |
Escherichia coli |
Cysteine--tRNA ligase | - |
Escherichia coli |
Cysteinyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Cysteinyl-transfer RNA synthetase | - |
Escherichia coli |
Cysteinyl-tRNA synthetase | - |
Escherichia coli |
Synthetase, cysteinyl-transfer ribonucleate | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |