Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
2 * 120000, SDS-PAGE in presence of 2-mercaptoethanol, enzyme form CRS-2 | Rattus norvegicus |
122000 | - |
gel filtration, enzyme form, CRS-1 | Rattus norvegicus |
127000 | - |
sucrose density gradient centrifugation, enzyme form CRS-2 | Rattus norvegicus |
230000 | 240000 | gel filtration, PAGE on gels of various porosities, enzyme form CRS-2 | Rattus norvegicus |
270000 | 300000 | gel filtration, enzyme form CRS-3 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
multiple molecular forms: CRS-1, CRS-2, CRS-3 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
enzyme form CRS-1 | Rattus norvegicus |
3.29 | - |
enzyme form CRS-2 | Rattus norvegicus |
6.25 | - |
enzyme form CRS-3 | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, 50% glycerol, 0.5 mM DTT, 3 mM L-cysteine | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Rattus norvegicus | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 120000, SDS-PAGE in presence of 2-mercaptoethanol, enzyme form CRS-2 | Rattus norvegicus |