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Literature summary for 6.1.1.16 extracted from

  • Pan, F.; Lee, H.H.; Pai, S.H.; Yu, T.C.; Guoo, J.Y.; Duh, G.M.
    Multiple molecular forms of cysteinyl-tRNA synthetase from rat liver: Purification and subunit structure (1976), Biochim. Biophys. Acta, 452, 271-283.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
2 * 120000, SDS-PAGE in presence of 2-mercaptoethanol, enzyme form CRS-2 Rattus norvegicus
122000
-
gel filtration, enzyme form, CRS-1 Rattus norvegicus
127000
-
sucrose density gradient centrifugation, enzyme form CRS-2 Rattus norvegicus
230000 240000 gel filtration, PAGE on gels of various porosities, enzyme form CRS-2 Rattus norvegicus
270000 300000 gel filtration, enzyme form CRS-3 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
multiple molecular forms: CRS-1, CRS-2, CRS-3 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
enzyme form CRS-1 Rattus norvegicus
3.29
-
enzyme form CRS-2 Rattus norvegicus
6.25
-
enzyme form CRS-3 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, 50% glycerol, 0.5 mM DTT, 3 mM L-cysteine Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
Rattus norvegicus AMP + diphosphate + L-cysteinyl-tRNACys
-
?

Subunits

Subunits Comment Organism
dimer 2 * 120000, SDS-PAGE in presence of 2-mercaptoethanol, enzyme form CRS-2 Rattus norvegicus