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Literature summary for 6.1.1.15 extracted from

  • Boyarshin, K.S.; Priss, A.E.; Rayevskiy, A.V.; Ilchenko, M.M.; Dubey, I.Y.; Kriklivyi, I.A.; Yaremchuk, A.D.; Tukalo, M.A.
    A new mechanism of post-transfer editing by aminoacyl-tRNA synthetases catalysis of hydrolytic reaction by bacterial-type prolyl-tRNA synthetase (2017), J. Biomol. Struct. Dyn., 35, 669-682 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)Star cells Enterococcus faecalis

Protein Variants

Protein Variants Comment Organism
G331A the mutant shows 6.7fold increased activity compared to the wild type enzyme Enterococcus faecalis
G334A the mutant shows 2.2fold increased activity compared to the wild type enzyme Enterococcus faecalis
H366A the mutant shows 4.4fold increased activity compared to the wild type enzyme Enterococcus faecalis
I263A the mutant shows 2.4fold increased activity compared to the wild type enzyme Enterococcus faecalis
I278A the mutant shows 12.8fold increased activity compared to the wild type enzyme Enterococcus faecalis
I333A the mutant shows 54.7fold increased activity compared to the wild type enzyme Enterococcus faecalis
K279A the mutant shows 2006.3fold increased activity compared to the wild type enzyme Enterococcus faecalis
S280A the mutant shows 2fold increased activity compared to the wild type enzyme Enterococcus faecalis
S332A the mutant shows 1.1fold increased activity compared to the wild type enzyme Enterococcus faecalis
T257A the mutant shows 1.1fold increased activity compared to the wild type enzyme Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-proline + tRNAPro Enterococcus faecalis
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q831W7
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Ni-NTA agarose column chromatography Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + L-tRNAAla
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAAla
-
?
ATP + L-proline + L-tRNAPro
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + L-tRNAProAla
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAProAla
-
?
ATP + L-proline + tRNAPro
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAPro
-
?

Synonyms

Synonyms Comment Organism
Prolyl-tRNA synthetase
-
Enterococcus faecalis
ProRS
-
Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Enterococcus faecalis