Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)Star cells | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
G331A | the mutant shows 6.7fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
G334A | the mutant shows 2.2fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
H366A | the mutant shows 4.4fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
I263A | the mutant shows 2.4fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
I278A | the mutant shows 12.8fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
I333A | the mutant shows 54.7fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
K279A | the mutant shows 2006.3fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
S280A | the mutant shows 2fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
S332A | the mutant shows 1.1fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
T257A | the mutant shows 1.1fold increased activity compared to the wild type enzyme | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Enterococcus faecalis | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q831W7 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Ni-NTA agarose column chromatography | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + L-tRNAAla | - |
Enterococcus faecalis | AMP + diphosphate + L-prolyl-tRNAAla | - |
? | |
ATP + L-proline + L-tRNAPro | - |
Enterococcus faecalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + L-tRNAProAla | - |
Enterococcus faecalis | AMP + diphosphate + L-prolyl-tRNAProAla | - |
? | |
ATP + L-proline + tRNAPro | - |
Enterococcus faecalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Prolyl-tRNA synthetase | - |
Enterococcus faecalis |
ProRS | - |
Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Enterococcus faecalis |