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Literature summary for 6.1.1.15 extracted from

  • Lipman, R.S.; Beuning, P.J.; Musier-Forsyth, K.; Hou, Y.M.
    Amino acid activation of a dual-specificity tRNA synthetase is independent of tRNA (2002), J. Mol. Biol., 316, 421-427.
    View publication on PubMed
Search for more literature for 6.1.1.15

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58635
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Methanocaldococcus jannaschii DSM 2661 Q58635
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNA in later work it is shown that cysteine is attached to tRNA(Pro) and thus constitutes a misaminoacylation event and not a dual specificity. Addition of tRNA has a small threefold stimulatory effect on cysteine activation. The lack of a major role of tRNA in activation of cysteine suggests that the dual specificity enzyme must distinguish cysteine from proline directly, without the assistance of each cognate tRNA, to achieve the necessary specificity required for protein synthesis Methanocaldococcus jannaschii AMP + diphosphate + L-cysteinyl-tRNA
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 ?
ATP + L-cysteine + tRNA in later work it is shown that cysteine is attached to tRNA(Pro) and thus constitutes a misaminoacylation event and not a dual specificity. Addition of tRNA has a small threefold stimulatory effect on cysteine activation. The lack of a major role of tRNA in activation of cysteine suggests that the dual specificity enzyme must distinguish cysteine from proline directly, without the assistance of each cognate tRNA, to achieve the necessary specificity required for protein synthesis Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + L-cysteinyl-tRNA
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 ?
ATP + L-proline + tRNAPro activation of proline does not require tRNA. The dual specificity enzyme must distinguish cysteine from proline directly, without the assistance of each cognate tRNA, to achieve the necessary specificity required for protein synthesis Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
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 ?
ATP + L-proline + tRNAPro activation of proline does not require tRNA. The dual specificity enzyme must distinguish cysteine from proline directly, without the assistance of each cognate tRNA, to achieve the necessary specificity required for protein synthesis Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + L-prolyl-tRNAPro
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 ?

Synonyms

Synonyms Comment Organism
Prolyl-tRNA synthetase
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 Methanocaldococcus jannaschii
ProRS
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 Methanocaldococcus jannaschii