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Literature summary for 6.1.1.15 extracted from
- Prevention of mis-aminoacylation of a dual-specificity aminoacyl-tRNA synthetase (2002), J. Mol. Biol., 315, 943-999.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
tRNAPro | pH 7.0, 37°C | Methanocaldococcus jannaschii |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii DSM 2661 | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | Q58635 | - |
- |
| Methanocaldococcus jannaschii DSM 2661 | Q58635 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | the unmodified transcript of Methanococcus jannaschii tRNAPro is mis-acylated with cysteine. The origin of mischarging is not at the anticodon or acceptor stem. Replacement of the D loop in the tRNA core with that of tRNACys suppresses mischarging with cysteine without compromising the activity of aminoacylation with proline. Prevention of mis-placement by alteration of the core structure or by nucleotide modifications in the tRNA illustrates a novel strategy of the dual-specificity synthetase | Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | the unmodified transcript of Methanococcus jannaschii tRNAPro is mis-acylated with cysteine. The origin of mischarging is not at the anticodon or acceptor stem. Replacement of the D loop in the tRNA core with that of tRNACys suppresses mischarging with cysteine without compromising the activity of aminoacylation with proline. Prevention of mis-placement by alteration of the core structure or by nucleotide modifications in the tRNA illustrates a novel strategy of the dual-specificity synthetase | Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay at | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
tRNAPro | pH 7.0, 37°C | Methanocaldococcus jannaschii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Methanocaldococcus jannaschii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19330 | - |
tRNAPro | pH 7.0, 37°C | Methanocaldococcus jannaschii | |
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