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Literature summary for 6.1.1.15 extracted from

  • Zhang, C.M.; Perona, J.J.; Ryu, K.; Francklyn, C.; Hou, Y.M.
    Distinct kinetic mechanisms of the two classes of aminoacyl-tRNA synthetases (2006), J. Mol. Biol., 361, 300-311.
    View publication on PubMed
Search for more literature for 6.1.1.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information single turnover and burst kinetics, steady-state kinetics, recombinant His-tagged enzyme, overview Deinococcus radiodurans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-proline + tRNAPro Deinococcus radiodurans
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro class II synthetases are limited by a step prior to aminoacyl transfer Deinococcus radiodurans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro
-
 Deinococcus radiodurans AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro a two-step reaction Deinococcus radiodurans AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

Synonyms Comment Organism
class II prolyl-tRNA synthetase
-
 Deinococcus radiodurans
class II ProRS
-
 Deinococcus radiodurans
Prolyl-tRNA synthetase
-
 Deinococcus radiodurans
ProRS
-
 Deinococcus radiodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Deinococcus radiodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 tRNAPro steady-state Deinococcus radiodurans
9.8
-
 tRNAPro single turnover overall aminoacylation Deinococcus radiodurans
10.8
-
 tRNAPro single turnover of the transfer step Deinococcus radiodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Deinococcus radiodurans

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Deinococcus radiodurans