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Literature summary for 6.1.1.15 extracted from
- A Comparison of the thermal stability and substrate binding constants of prolyl-tRNA synthetase from Phaseolus aureus and Delonix regia (1973), Phytochemistry, 12, 2109-2121.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| above 60°C, little protection by bovine serum albumin and glycerol | Phaseolus vulgaris |
| at pH 6.5, 0.01 mM Mg2+ stabilizes against thermal inactivation | Delonix regia |
| at pH 8, in absence of Mg2+, ATP does not protect against thermal inactivation | Phaseolus vulgaris |
| at pH 8, in absence of Mg2+, ATP does not protect against thermal inactivation | Delonix regia |
| bovine serum albumin and glycerol partially protect against thermal denaturation at 45°C and 50°C respectively | Phaseolus vulgaris |
| bovine serum albumin and glycerol partially protect against thermal denaturation at 45°C and 50°C respectively | Delonix regia |
| furan and tetrahydrothiophen, only in presence of ATP protect against thermal inactivation | Phaseolus vulgaris |
| furan and tetrahydrothiophen, only in presence of ATP protect against thermal inactivation | Delonix regia |
| mercaptoethanol or DTT stabilizes against heat inactivation | Delonix regia |
| Pro or ATP markedly stabilizes against heat inactivation | Phaseolus vulgaris |
| Pro or ATP markedly stabilizes against heat inactivation | Delonix regia |
| several analogues of Pro protect against thermal inactivation | Phaseolus vulgaris |
| several analogues of Pro protect against thermal inactivation | Delonix regia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Delonix regia | - |
- |
- |
| Phaseolus vulgaris | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-proline + tRNAPro | - |
Phaseolus vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |  |
| ATP + L-proline + tRNAPro | - |
Delonix regia | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
above 60°C, little protection by bovine serum albumin and glycerol | Phaseolus vulgaris |
| additional information | - |
bovine serum albumin and glycerol partially protect against thermal denaturation at 45°C and 50°C respectively | Phaseolus vulgaris |
| additional information | - |
bovine serum albumin and glycerol partially protect against thermal denaturation at 45°C and 50°C respectively | Delonix regia |
| additional information | - |
several analogues of Pro protect against thermal inactivation | Phaseolus vulgaris |
| additional information | - |
several analogues of Pro protect against thermal inactivation | Delonix regia |
| additional information | - |
mercaptoethanol or DTT stabilizes against heat inactivation | Delonix regia |
| additional information | - |
Pro or ATP markedly stabilizes against heat inactivation | Delonix regia |
| additional information | - |
at pH 6.5, 0.01 mM Mg2+ stabilizes against thermal inactivation | Delonix regia |
| additional information | - |
furan and tetrahydrothiophen, protect only in presence of ATP against thermal inactivation | Phaseolus vulgaris |
| additional information | - |
furan and tetrahydrothiophen, protect only in presence of ATP against thermal inactivation | Delonix regia |
| additional information | - |
at pH 8, in absence of Mg2+, ATP does not protect against thermal inactivation | Phaseolus vulgaris |
| additional information | - |
at pH 8, in absence of Mg2+, ATP does not protect against thermal inactivation | Delonix regia |
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