Protein Variants | Comment | Organism |
---|---|---|
C443A | mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold | Escherichia coli |
C443G | mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold | Escherichia coli |
C443S | mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | more than 90% protection by 10 mM ATP or 10 mM ATP + 10 mM Pro | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Pro | wild-type | Escherichia coli | |
1.7 | - |
Pro | mutant C443A | Escherichia coli | |
2.4 | - |
Pro | mutant C443G | Escherichia coli | |
3.1 | - |
Pro | mutant C443S | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type enzyme and mutant enzymes C443A, C443G, and C443S | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
ATP | mutant C443S, ATP-diphosphate exchange | Escherichia coli | |
15 | - |
ATP | mutant C443G, ATP-diphosphate exchange | Escherichia coli | |
28 | - |
ATP | mutant C443A, ATP-diphosphate exchange | Escherichia coli | |
81 | - |
ATP | wild-type enzyme, ATP-diphosphate exchange | Escherichia coli |