Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain InvSc1 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
tRNAGly | wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.00073 | - |
tRNAGly | wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.00137 | - |
tRNAGly | wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.13 | - |
glycine | wild type isoform GlyRS1(KMM1), in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.135 | - |
glycine | wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.14 | - |
glycine | wild type isoform GlyRS1 without K motif, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.145 | - |
glycine | wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.145 | - |
glycine | wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | Saccharomyces cerevisiae | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Saccharomyces cerevisiae ATCC 204508 | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38088 | mitochondrial isoform GlyRS1 | - |
Saccharomyces cerevisiae | Q06817 | isoform GlyRS2 | - |
Saccharomyces cerevisiae ATCC 204508 | P38088 | mitochondrial isoform GlyRS1 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | - |
Saccharomyces cerevisiae | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Saccharomyces cerevisiae ATCC 204508 | AMP + diphosphate + glycyl-tRNAGly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glycyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
GlyRS | - |
Saccharomyces cerevisiae |
GlyRS1 | isoform | Saccharomyces cerevisiae |
GlyRS2 | isoform | Saccharomyces cerevisiae |
GRS1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
tRNAGly | wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.33 | - |
tRNAGly | wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
0.73 | - |
tRNAGly | wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
tRNAGly | wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
220 | - |
tRNAGly | wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae | |
1230 | - |
tRNAGly | wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C | Saccharomyces cerevisiae |