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Literature summary for 6.1.1.14 extracted from

  • Duchene, A.M.; Peeters, N.; Dietrich, A.; Cosset, A.; Small, I.D.; Wintz, H.
    Overlapping destinations for two dual targeted glycyl-tRNA synthetases in Arabidopsis thaliana and Phaseolus vulgaris (2001), J. Biol. Chem., 276, 15275-15283.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of isozyme GlyRS1 in Escherichia coli strain TG2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme GlyRS2 Phaseolus vulgaris 9507
-
chloroplast isozyme GlyRS2 Arabidopsis thaliana 9507
-
cytosol isozyme GlyRS1, active Phaseolus vulgaris 5829
-
cytosol isozyme GlyRS1, active Arabidopsis thaliana 5829
-
mitochondrion isozyme GlyRS2, enzyme is imported from the cytosol, nucleus-encoded Arabidopsis thaliana 5739
-
mitochondrion isozymes GlyRS1 and GlyRS2, enzymes are imported from the cytosol, nucleus-encoded, isozyme GlyRS1 is inactive in the mitochondria Phaseolus vulgaris 5739
-
mitochondrion isozymes GlyRS1, enzyme is imported from the cytosol, nucleus-encoded, isozyme GlyRS1 is inactive in the mitochondria Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE Arabidopsis thaliana
30000
-
4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE Arabidopsis thaliana
80000
-
isozyme GlyRS1 Arabidopsis thaliana
117000
-
isozyme GlyRS2 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycine + tRNAGly Phaseolus vulgaris
-
AMP + diphosphate + glycyl-tRNAGly
-
?
ATP + glycine + tRNAGly Arabidopsis thaliana
-
AMP + diphosphate + glycyl-tRNAGly
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O23627 2 dual-targeted isozymes
-
Arabidopsis thaliana Q8L785 2 dual-targeted isozymes
-
Phaseolus vulgaris
-
2 dual-targeted isozymes
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme GlyRS1 from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
Phaseolus vulgaris AMP + diphosphate + glycyl-tRNAGly
-
?
ATP + glycine + tRNAGly
-
Arabidopsis thaliana AMP + diphosphate + glycyl-tRNAGly
-
?
ATP + glycine + tRNAGly chloroplastic and mitochondrial tRNA substrate specificity of chloroplastic isozyme GlyRS2, isozyme GlyRS1 is much less efficient Phaseolus vulgaris AMP + diphosphate + glycyl-tRNAGly
-
?

Subunits

Subunits Comment Organism
dimer or trimer 2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE Arabidopsis thaliana
tetramer 4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Glycine--tRNA ligase
-
Phaseolus vulgaris
Glycine--tRNA ligase
-
Arabidopsis thaliana
Glycyl translase
-
Phaseolus vulgaris
Glycyl translase
-
Arabidopsis thaliana
Glycyl-transfer ribonucleate synthetase
-
Phaseolus vulgaris
Glycyl-transfer ribonucleate synthetase
-
Arabidopsis thaliana
Glycyl-transfer ribonucleic acid synthetase
-
Phaseolus vulgaris
Glycyl-transfer ribonucleic acid synthetase
-
Arabidopsis thaliana
Glycyl-transfer RNA synthetase
-
Phaseolus vulgaris
Glycyl-transfer RNA synthetase
-
Arabidopsis thaliana
Glycyl-tRNA synthetase
-
Phaseolus vulgaris
Glycyl-tRNA synthetase
-
Arabidopsis thaliana
GlyRS
-
Phaseolus vulgaris
GlyRS
-
Arabidopsis thaliana
Synthetase, glycyl-transfer ribonucleate
-
Phaseolus vulgaris
Synthetase, glycyl-transfer ribonucleate
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Phaseolus vulgaris
ATP
-
Arabidopsis thaliana